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ID   MINIF      preliminary; circular DNA; SYN; 192 BP.
AC   M12680;
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector mini-F - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-192
RC   from pEB100
RC   from pEB100Cam5
RC   from pMBO80
RA   Miller J.F., Malamy M.H.;
RT   "Mutational and in vivo methylation analysis of F-factor PifC
RT   protein binding to the pif operator and the region containing the
RT   primary origin of mini-F replication";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:1433-1437(1986).
RN   [2]
RC   pEB100 from pBR322 & pGS103
RC   pEB100Cam5 from pEB100 & pifC5(Am) region
RA   Buchert E.;
RT   ;
RL   Unpublished (1986).
RN   [3]
RC   pMBO80 from pBR322 & pGS103
RA   O'Connor M.B., Malamy M.H.;
RT   "Site-specific recombination in the oriV1 region of the F factor";
RL   Cold Spring Harb. Symp. Quant. Biol. 49:421-434(1984).
RN   [4]
RC   pJH3 from pRS5
RC   pKC17 from pRS5
RC   pKC25 from pRS5
RC   pKC43 from pRS5
RC   pST11 from pRS5
RC   pGS103 from pJH3 & pKC43
RC   pGS204 from pGS103
RC   pGS211 from pGS103
RC   pGS221 from pGS103
RC   pGS21, pGS35 from pGS103
RC   pVU14 from pGS103
RC   from pRS5 & Tn5
RA   Rotman G.S., Cooney R., Malamy M.H.;
RT   "Cloning of the pif region of the F sex factor and identification
RT   of a pif protein product";
RL   J. Bacteriol. 155:254-264(1983).
RN   [5]
RC   pJM1383 from pSC138 & pGS103 & F factor, pif region
RA   Miller J.F., Malamy M.H.;
RT   "Regulation of the F-factor pif operon: pifO, a site required in
RT   cis for autoregulation, titrates the pifC product in trans";
RL   J. Bacteriol. 160:192-198(1984).
RN   [6]
RC   pRC201 from pMC1403 & pGS103
RA   Cooney R.;
RT   ;
RL   Unpublished (1984).
RN   [7]
RC   pJM521 from lacZ
RC   F' C521 from pJM521 & F factor, pif region
RC   F' lac from F factor
RC   F' lac C21 from F factor
RC   [mini-F from F factor, pif region]
RA   Miller J.F., Malamy M.H.;
RT   "Identification of the pifC gene and its role in negative control
RT   of F factor pif gene expression";
RL   J. Bacteriol. 156:338-347(1983).
CC   NCBI gi: 209021
CC   NM (mini-F)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. F'lac EcoRI-EcoRI 9100bp, f5 frag/40.3-49.4
FT                   -> mini-F 9100bp"
FT   rep_origin      0..0
FT                   /note="ORI mini-F"
SQ   Sequence 192 BP; 53 A; 35 C; 26 G; 78 T; 0 other;
     catcatcgat cctttaatgt ttgttttgtt cttaatgcta acctagcatt ttattattag
     ctattaatca gttttttagt ttttgaaaat tgtgatgctt ctaaaattac taaaattgag
     gattttaatg ctacaacaat gcctgcctct tcttatttct ccggagatcc gaaaacccca
     agttacggat ct