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ID   MTN        preliminary; circular DNA; SYN; 7900 BP.
AC   IG7025;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector MT-N - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   MT-N from MMLV LTR & mouse MT promoter & neo gene
RC   SV-N from MMLV LTR & SV40 early promoter & neo gene
RC   TK-N from MMLV LTR & HSV tk gene promoter & neo gene
RA   Yu S., Von Ruden T., Kantoff P.W., Garber C., Seiberg M., Ruther U.,
RA   Wagner E., Anderson W.F., Gilboa E.;
RT   "Self-inactivating retroviral vectors designed for transfer of whole
RT   genes into mammalian cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:3194-3198(1986).
CC   NM (MT-N)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pMT)(pSP64)
CC   BR ()
CC   OF (pMR8 also from pYN)(pMR9 also from pYN)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. MMLV remove PvuII-SacI 299bp, 3'LTR enhancer
FT                   -> virus
FT                   1. MMLV left end-BamHI, 5' LTR
FT                   2. mouse BamHI-, MT promoter
FT                   3. E. coli -XbaI, neo gene
FT                   4. virus ClaI-right end, 140bp 5' to 3' LTR
FT                   -> MT-N"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    0..0
FT                   /note="RPT human metallothionein gene long terminal
FT                   repeat, LTR region"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-HindIII-EcoRI-XhoI-NotI-MluI-StuI-
FT                   XbaI"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;