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ID   NT4        preliminary; circular DNA; SYN; 171 BP.
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AC   D16588;
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DT   12-AUG-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli phage vector NT4 - incomplete, MCS.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-171
RC   NT4, NT5 from oligo
RC   pNST4, pNST5 from pBR322
RA   Nomura N., Tabata S.;
RT   "A simple method for constructing cloning vehicles with long
RT   polylinker sequences";
RL   Bull. Inst. Chem. Res., Kyoto Univ. 71:252-258(1993).
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RN   [2]
RP   1-171
RC   NT4
RA   Nomura N.;
RT   ;
RL   Submitted (02-JUL-1993) to DDBJ by:
RL   Nomura N., Dept. of Molecular Biology, Nippon Medical School,
RL   Institute of Gerontology, 1-396 Kosugumachi, Nakahara-ku, Kawasaki,
RL   Kanagawa 211, JAPAN.
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CC   NCBI gi: 391914
CC   NM (NT4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. oligo, MCS/ori
FT                   -> NT4"
FT   misc_feature    1..171
FT                   /note="polylinker"
FT   rep_origin      0..0
FT                   /note="ORI NT4"
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SQ   Sequence 171 BP; 42 A; 46 C; 44 G; 39 T; 0 other;
     caggaaacag ctatgaccat gattacgaat tcgaagctta aggcctccat ggatcccggg
     tcgacgcgta cgatatcgat gtctagatct ccagtactag tctcgagctc tgcagggccc
     gcggtaccat gcatactggc cgtcgtttta caacgtcgtg actgggaaaa c
//