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ID   P219       preliminary; circular DNA; SYN; 8800 BP.
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AC   ATCC39550;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector p219 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   p219 from pBR322 & yeast 2 micron
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,703,004 dated Oct. 27, 1987.
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RN   [2]
RC   p219 from pBR322 & yeast 2 micron
RA   ;
RT   ;
RL   Unpublished (1988).
RL   U.S. Patent No. 4,782,137 dated Nov. 1, 1988.
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CC   An EcoRI/HindIII double digest will give a 7.4 kb fragment of the
CC   vector retaining all the vector functions.  The EcoRI site is just
CC   downstream of the yeast promoter. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--8.8; PstI--7.2, 1.6; HindIII--6.6, 2.2; XbaI--6.85, 1.95. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (p219)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI 4361bp 4360..4360
FT                   2. yeast 2 micron EcoRI-EcoRI 2406bp 2..2408, ori
FT                   3. yeast 2000bp, promoter
FT                   -> p219 8800bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO yeast gene; unspecified"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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