back Return to this vector's summary.
ID   P366       preliminary; circular DNA; SYN; 8479 BP.
AC   ATCC77163;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector p366 or pBS32 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pYCF5 from YIp series & SUP11 gene & URA3 gene
RA   Hegemann J.H., Shero J.H., Cottarel G., Philippsen P., Hieter P.;
RT   "Mutational analysis of centromere DNA from chromosomeVI of
RT   Sacchraomyces cerevisiae";
RL   Mol. Cell. Biol. 8:2523-2535(1988).
RN   [2]
RC   pMS191 from histone H4 ARS
RA   Smith M.M., Murray K.;
RT   "Yeast H3 and H4 histone messenger RNAs are transcribed from two
RT   non-allelic gene sets";
RL   J. Mol. Biol. 169:641-661(1983).
RN   [3]
RC   p366 or pBS32 from YCp50 & LEU2 gene
RA   Trueheart J.;
RT   ;
RL   Unpublished (1989).
CC   Derived from YCp50 (ATCC 37419) by removing most of the URA3 gene as a
CC   1.89 kb SalI/SmaI fragment and replacing it with a 2.23 kb SalI/XhoI
CC   fragment containing LEU2. (personal communication)
CC   YC-type shuttle vector. (personal communication)
CC   P. Hieter <-- J. Trueheart. (personal communication)
CC   The order of the major features in this plasmid is:HindIII - BamHI -
CC   SphI - LEU2 - CEN4 - ARS1 - pMB1 ori - bla. (personal communication)
CC   This vector was constructed by Josh Trueheart. (personal
CC   communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--6.5, 2.0; BamHI--8.5; HindIII--8.5; BglII--8.5;
CC   BamHI/BglII--5.6, 2.9. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (p366)(pBS32)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. YCp50 remove SalI-SmaI 1890bp 655..2543,
FT                   URA3 gene
FT                   2. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   -> p366 8479bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-BamHI-SphI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN4"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;