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ID   P826B      preliminary; circular DNA; SYN; 15143 BP.
XX
AC   IG0013;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector p82-6B - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   p82-6B from pBR322 & yeast 2 micron
RA   ;
RT   ;
RL   Unpublished (1992).
XX
CC   NM (p82-6B)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(yeast)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(yeast 2 micron)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
XX
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/tet gene
FT                   2. yeast 2 micron 6318bp, ori
FT                   3. yeast 4464bp, FLP/REP1/REP2 genes
FT                   -> p82-6B 15143bp"
FT   CDS             0..0
FT                   /note="GEN Saccharomyces FLP gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322);
FT                   similar to it"
FT   CDS             887..2008
FT                   /note="GEN Saccharomyces REP1 gene"
FT   CDS             7124..8314
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   rep_origin      9595..9945
FT                   /note="ORI yeast 2 micron"
FT   CDS             13203..14093
FT                   /note="GEN Saccharomyces REP2 gene"
XX
SQ   Sequence 15143 BP; 2955 A; 2251 C; 2101 G; 3017 T; 4819 other;
     ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa tgcgccttat
     tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt gatgacctca
     tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg gagcgataag
     cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat acctgatcac
     tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga aatgatagca
     ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct aaagggtagt
     gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac
     tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata aacacgcact
     atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga
     acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac
     gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact
     gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc
     tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt
     gaacttgcat ctaaactcga cctctacatc aacaggcttc caatgctctt caaattttac
     tgtcaagtag acccatacgg ctgtaatatg ctgctcttca taatgtaagc ttatctttat
     cgaatcgtgt gaaaaactac taccgcgata aacctttacg gttccctgag attgaattag
     ttcctttagt atatgataca agacactttt gaactttgta cgacgaattt tgaggttcgc
     catcctctgg ctatttccaa ttatcctgtc ggctattatc tccgcctcag tttgatcttc
     cgcttcagac tgccattttt cacataatga atctatttca ccccacaatc cttcatccgc
     ctccgcatct tgttccgtta aactattgac ttcatgttgt acattgttta gttcacgaga
     agggtcctct tcaggcggta gctcctgatc tcctatatga cctttatcct gttctctttc
     cacaaactta gaaatgtatt catgaattat ggagcaccta ataacattct tcaaggcgga
     gaagtttggg ccagatgccc aatatgcttg acatgaaaac gtgagaatga atttagtatt
     attgtgatat tctgaggcaa ttttattata atctcgaaga taagagaaga atgcagtgac
     ctttgtattg acaaatggag attccatgta tctaaaaaat acgcctttag gccttctgat
     accctttccc ctgcggttta gcgtgccttt tacattaata tctaaaccct ctccgatggt
     ggcctttaac tgactaataa atgcaaccga tataaactgt gataattctg ggtgatttat
     gattcgatcg acaattgtat tgtacactag tgcaggatca ggccaatcca gttctttttc
     aattaccggt gtgtcgtctg tattcagtac atgtccaaca aatgcaaatg ctaacgtttt
     gtatttctta taattgtcag gaactggaaa agtccccctt gtcgtctcga ttacacacct
     actttcatcg tacaccatag gttggaagtg ctgcataata cattgcttaa tacaagcaag
     cagtctctcg ccattcatat ttcagttatt ttccattaca gctgatgtca ttgtatatca
     gcgctgtaaa aatctatctg ttacagaagg ttttcgcggt ttttataaac aaaactttcg
     ttacgaaatc gagcaatcac cccagctgcg tatttggaaa ttcgggaaaa agtagagcaa
     cgcgagttgc attttttaca ccataatgca tgattaactt cgagaaggga ttaaggctaa
     tttcactagt atgtttcaaa aacctcaatc tgtccattga atgccttata aaacagctat
     agattgcata gaagagttag ctactcaatg ctttttgtca aagcttactg atgatgatgt
     gtctactttc aggcgggtct gtagtaagga gaatgacatt ataaagctgg cacttagaat
     tccacggact atagactata ctagtatact ccgtctactg tacgatacac ttccgctcag
     gtccttgtcc tttaacgagg ccttaccact cttttgttac tctattgatc cagctcagca
     aaggcagtgt gatctaagat tctatcttcg cgatgtagta aaactagcta gaccgagaaa
     gagactagaa atgcaaaagg cacttctaca atggctgcca tcattattat ccgatgtgac
     gctgcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
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     nnnnnnnnnn nnnnnnnnnn nnnnnctcgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
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     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnngttg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
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     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
     ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
     tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc
     accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa
     cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat
     cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc
     tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag
     cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
     accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
     gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
     cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
     gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg
     ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
     caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
     ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     gatgccggcc acgatgcgtc cggcgtagag gatccacagg acgggtgtgg tcgccatgat
     cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg
     gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag
     cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg
     cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac
     gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga
     taaactaccg cattaaacag aattctgaac cagtcctaaa acgagtaaat aggaccggca
     agcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
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     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaattc ttcaagcaat
     aaacaggaat accaattatt aaaagataac ttagtcagat cgtacaataa agctttgaag
     aaaaatgcgc cttattcaat ctttgctata aaaaatggcc caaaatctca cattggaaga
     catttgatga cctcatttct ttcaatgaag ggcctaacgg agttgactaa tgttgtggga
     aattggagcg ataagcgtgc ttctgccgtg gccaggacaa cgtatactca tcagataaca
     gcaatacctg atcactactt cgcactagtt tctcggtact atgcatatga tccaatatca
     aaggaaatga tagcattgaa ggatgagact aatccaattg aggagtggca gcatatagaa
     cagctaaagg gtagtgctga aggaagcata cgataccccg catggaatgg gataatatca
     caggaggtac tagactacct ttcatcctac ataaatagac gcatataagt acgcatttaa
     gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat
     ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg
     ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct agaaagtata
     ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa gacgcacttt caaaaaacca
     aaaacgcacc ggactgtaac gagctactaa aatattgcga ataccgcttc cacaaacatt
     gctcaaaagt atctctttgc tatatatctc tgtgctatat ccctatataa cctacccatc
     cacctttcgc tccttgaact tgcatctaaa ctcgacctct acatcaacag gcttccaatg
     ctcttcaaat tttactgtca agtagaccca tacggctgta atatgctgct cttcataatg
     taagcttatc tttatcgaat cgtgtgaaaa actactaccg cgataaacct ttacggttcc
     ctgagattga attagttcct ttagtatatg atacaagaca cttttgaact ttgtacgacg
     aattttgagg ttcgccatcc tctggctatt tccaattatc ctgtcggcta ttatctccgc
     ctcagtttga tcttccgctt cagactgcca tttttcacat aatgaatcta tttcacccca
     caatccttca tccgcctccg catcttgttc cgttaaacta ttgacttcat gttgtacatt
     gtttagttca cgagaagggt cctcttcagg cggtagctcc tgatctccta tatgaccttt
     atcctgttct ctttccacaa acttagaaat gtattcatga attatggagc acctaataac
     attcttcaag gcggagaagt ttgggccaga tgcccaatat gcttgacatg aaaacgtgag
     aatgaattta gtattattgt gatattctga ggcaatttta ttataatctc gaagataaga
     gaagaatgca gtgacctttg tattgacaaa tggagattcc atgtatctaa aaaatacgcc
     tttaggcctt ctgataccct ttcccctgcg gtttagcgtg ccttttacat taatatctaa
     accctctccg atggtggcct ttaactgact aataaatgca accgatataa actgtgataa
     ttctgggtga tttatgattc gatcgacaat tgtattgtac actagtgcag gatcaggcca
     atccagttct ttttcaatta ccggtgtgtc gtctgtattc agtacatgtc caacaaatgc
     aaatgctaac gttttgtatt tcttataatt gtcaggaact ggaaaagtcc cccttgtcgt
     ctcgattaca cacctacttt catcgtacac cataggttgg aagtgctgca taatacattg
     cttaatacaa gcaagcagtc tctcgccatt catatttcag ttattttcca ttacagctga
     tgtcattgta tatcagcgct gtaaaaatct atctgttaca gaaggttttc gcggttttta
     taaacaaaac tttcgttacg aaatcgagca atcaccccag ctgcgtattt ggaaattcgg
     gaaaaagtag agcaacgcga gttgcatttt ttacaccata atgcatgatt aacttcgaga
     agggattaag gctaatttca ctagtatgtt tcaaaaacct caatctgtcc attgaatgcc
     ttataaaaca gctatagatt gcatagaaga gttagctact caatgctttt tgtcaaagct
     tactgatgat gatgtgtcta ctttcaggcg ggtctgtagt aaggagaatg acattataaa
     gctggcactt agaattccac ggactataga ctatactagt atactccgtc tactgtacga
     tacacttccg ctcaggtcct tgtcctttaa cgaggcctta ccactctttt gttactctat
     tgatccagct cagcaaaggc agtgtgatct aagattctat cttcgcgatg tagtaaaact
     agctagaccg agaaagagac tagaaatgca aaaggcactt ctacaatggc tgccatcatt
     attatccgat gtgacgctgc agcttctcaa tgatattcga atacgctttg aggagataca
     gcctaatatc cgacaaactg ttttacagat ttacgatcgt acttgttacc catcattgaa
     ttttgaacat ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata
     ataatatata gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac
     tattgcatct attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt
     ccatcttgca cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga
     acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
     tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
     actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
     gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
     tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
     aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
     gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
     ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
     aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
     gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
     ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
     attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
     tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata
     ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
     gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
     tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat
     gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca
     tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc
     ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcataccc
     tagaagtatt acgtgatttt ctgcccctta ccctcgttgc tactctcctt tttttcgtgg
     gaaccgcttt agggccctca gtgatggtgt tttgtaattt atatgctcct cttgcatttg
     tgtctctact tcttgttcgc ctggagggaa cttcttcatt tgtattagca tggttcactt
     cagtccttcc ttccaactca ctcttttttt gctgtaaacg attctctgcc gccagttcat
     tgaaactatt gaatatatcc tttagagatt ccgggatgaa taaatcacct attaaagcag
     cttgacgatc tggtggaact aaagtaagca attgggtaac gacgcttacg agcttcataa
     catcttcttc cgttggagct ggtgggacta ataactgtgt acaatccatt tttctcatga
     gcatttcggt agctctcttc ttgtctttct cgggcaatct tcctattatt atagcaatag
     atttgtatag ttgctttcta ttgtctaaca gcttgttatt ctgtagcatc aaatctatgg
     cagcctgact tgcttcttgt gaagagagca taccatttcc aatcgaatca aacctttcct
     taaccatctt cgcagcaggc aaaattacct cagcactgga gtcagaagat acgctggaat
     cttctgcgct agaatcaaga ccatacggcc taccggttgt gagagattcc atgggcctta
     tgacatatcc tggaaagagt agctcatcag acttacgttt actctctata tcaatatcta
     catcaggagc aatcatttca ataaacagcc gacatacatc ccagacgcta taagctgtac
     gtgcttttac cgtcagattc ttggctgttt caatgtcgtc cattttggtt ttcttttacc
     agtattgttc gtttgataat gtattcttgc ttattacatt ataaaatctg tgcagatcac
     atgtcaaaac aactttttat cacaagatag taccgcaaaa cgaacctgcg ggccgtctaa
     aaattaagga aaagcagcaa aggtgcattt ttaaaatatg aaatgaagat accgcagtac
     caattatttt cgcagtacaa ataatgcgcg gccggtgcat ttttcgaaag aacgcgagac
     aaacaggaca attaaagtta gtttttcgag ttagcgtgtt tgaatactgc aagatacaag
     ataaatagag tagttgaaac tagatatcaa ttgcacacaa gatcggcgct aagcatgcca
     caatttggta tattatgtaa aacaccacct aaggtgcttg ttcgtcagtt tgtggaaagg
     tttgaaagac cttcaggtga gaaaatagca ttatgtgctg ctgaactaac ctatttatgt
     tggatgatta cacataacgg aacagcaatc aagagagcca cattcatgag ctataatact
     atcataagca attcgctgag tttcgatatt gtcaataaat cactccagtt taaatacaag
     acgcaaaaag caacaattct ggaagcctca ttaaagaaat tgattcctgc ttgggaattt
     acaattattc cttactatgg acaaaaacat caatctgata tcactgatat tgtaagtagt
     ttgcaattac agttcgaatc atcggaagaa gcagataagg gaaatagcca cagtaaaaaa
     atgcttaaag cacttctaag tgagggtgaa agcatctggg agatcactga gaaaatacta
     aattcgtttg agtatacttc gagatttaca aaaacaaaaa ctttatacca attcctcttc
     ctagctactt tcatcaattg tggaagattc agcgatatta agaacgttga tccgaaatca
     tttaaattag tccaaaataa gtatctggga gtaataatcc agtgtttagt gacagagaca
     aagacaagcg ttagtaggca catatacttc tttagcgcaa ggggtaggat cgatccactt
     gtatatttgg atgaattttt gag
//