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ID   PAA31      preliminary; circular DNA; SYN; 8900 BP.
AC   ATCC37309;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pAA31 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pAA3 from pBR322 & lambda gal+
RC   pAA31 from pBR322 & lambda galc200
RC   pAA31H from pAA31
RC   pAA31P from pAA31 & Tn9
RC   pAA3H from pAA3
RA   Ahmed A.;
RT   "Plasmid vectors for positive galactose-resistance selection of
RT   cloned DNA in Escherichia coli";
RL   Gene 28:37-43(1984).
RN   [2]
RC   lambda gal3 from lambda
RC   lambda gal+ from lambda gal3
RC   lambda galc31 from lambda gal3
RC   lambda galc33 from lambda gal3
RC   lambda galc331 (chlD-pgl) from lambda gal3
RC   lambda galc200 from lambda gal3
RA   Ahmed A., Scraba D.;
RT   "Nature of deletions formed in response to IS2 in a revertant of
RT   the gal3 insertion of E. coli";
RL   Mol. Gen. Genet. 163:189-196(1978).
CC   Vector for positive galactose-resistance selection of DNA cloned into
CC   the HindIII site.
CC   A HindIII/BamHI fragment (approximately 3.0 kb) containing galE'TK,
CC   and a BamHI/EcoRI fragment containing lambda att, were inserted into
CC   the HindIII/EcoRI sites of pBR322.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAA31)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli delta4)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda 48502bp
FT                   2. E. coli, gal3 gene [gal with IS2 1100bp]
FT                   -> lambda gal3 49000bp
FT                   1. lambda gal3 remove chlD-pgl region
FT                   -> lambda gal+ 48000bp
FT                   1. pBR322 remove EcoRI-HindIII 31bp 4360..4361..30,
FT                   \ 4330bp
FT                   2. lambda gal+ HindIII-EcoRI 8940bp, gal operon
FT                   -> pAA3 13270bp
FT                   1. pBR322 HindIII-EcoRI 31bp 4360..4361..30
FT                   2. lambda galc200 HindIII-BamHI, gal operon
FT                   3. lambda galc200 BamHI-EcoRI, att
FT                   -> pAA31 8880bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-HindIII-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase gene (galE'TK);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase galS gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;