back Return to this vector's summary.
ID   PAA31P     preliminary; circular DNA; SYN; 9200 BP.
XX
AC   ATCC37310;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pAA31P - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pAA3 from pBR322 & lambda gal+
RC   pAA31 from pBR322 & lambda galc200
RC   pAA31H from pAA31
RC   pAA31P from pAA31 & Tn9
RC   pAA3H from pAA3
RA   Ahmed A.;
RT   "Plasmid vectors for positive galactose-resistance selection of
RT   cloned DNA in Escherichia coli";
RL   Gene 28:37-43(1984).
XX
CC   Vector for positive galactose-resistance selection of DNA cloned into
CC   the HindIII site.
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pAA31P)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli delta4)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAA31 remove small PstI-PstI 3614..?,
FT                   \ amp to lambda
FT                   2. Tn9 PstI-PstI 1870bp, amp gene
FT                   -> pAA31P 9200bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuI-HpaI-SalI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase gene (galE'TK);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase galS gene"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//