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ID   PAA3H      preliminary; circular DNA; SYN; 11500 BP.
AC   ATCC37308;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli cosmid vector pAA3H - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pAA3 from pBR322 & lambda gal+
RC   pAA31 from pBR322 & lambda galc200
RC   pAA31H from pAA31
RC   pAA31P from pAA31 & Tn9
RC   pAA3H from pAA3
RA   Ahmed A.;
RT   "Plasmid vectors for positive galactose-resistance selection of
RT   cloned DNA in Escherichia coli";
RL   Gene 28:37-43(1984).
CC   Vector for positive galactose-resistance selection of DNA cloned into
CC   the HindIII site.
CC   The galE'TK genes are carried on a HindIII/HpaI fragment of
CC   approximately 3.0 kb. The galE gene is truncated at the HindIII site.
CC   Expression of the gal sequences are regulated by a promoter located
CC   between the BamHI and HindIII sites in the tet gene.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAA3H)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli delta4)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda 48502bp
FT                   2. E. coli gal3 gene [gal with IS2 1100bp]
FT                   -> lambda gal3 49000bp
FT                   1. lambda gal3 remove chlD-pgl region
FT                   -> lambda gal+ 48000bp
FT                   1. pBR322 remove EcoRI-HindIII 31bp 4360..4361..30,
FT                   \ 4330bp
FT                   2. lambda gal+ HindIII-EcoRI 8940bp, gal operon
FT                   -> pAA3 13270bp
FT                   1. pAA3 remove HpaI-HpaI 1760bp, lambda
FT                   -> pAA3H 11510bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaI-BglII-ClaI-EcoRI-Tth111I-
FT                   BalI-SalI-BamHI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase gene (galE'TK);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase galS gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;