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ID   PAB3662    preliminary; circular DNA; SYN; 4400 BP.
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AC   ATCC77135;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Aspergillus/Neurospora/E.coli plasmid vector pAB366-2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAB366 or pAMPh366 from pRMH200 & pAm200
RC   pAB366-1 from pAB366
RC   pAB366-2 from pAB366 & pTZ19R
RC   pAB520 or pAmPh520 from pUC18 & pAB366-1
RC   pAB366Ben from pAB366 & pBT6
RA   Austin B., Hall R.M., Tyler B.M.;
RT   "Optimized vectors and selection for transformation of Neurospora
RT   crassa and Aspergillus nidulans to bleomycin and phleomycin
RT   resistance";
RL   Gene 93:157-162(1990).
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CC   Differs from pAB366 (ATCC 77134) by the insertion of a NsiI cloning
CC   site in the 5' untranslated region of ble to permit transcriptional
CC   fusion.
CC   The order of the major features in this plasmid is:pTZ19R - EcoRI -
CC   BamHI - am promoter - NsiI - ble - XhoI - am 3' region - HindIII -
CC   pTZ19R.
CC   Shuttle vector conferring bleomycin and phleomycin resistance.
CC   Expression of ble (bleomycin resistance) is regulated by the promoter
CC   and terminator regions of the am gene (amination, glutamate
CC   dehydrogenase) from N. crassa.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI/HindIII--2.9,1.5; BamHI/XhoI--3.6, 0.8; BglI--3.0, 1.4. (ATCC
CC   staff)
CC   A (GT)n repeat. (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAB366-2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Aspergillus nidulans)(Neurospora crassa)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAB366 4200bp
FT                   mutagenesis [makes NsiI in 5' UTR am gene]
FT                   EcoRI-HindIII, pUC8 MCS 231..-am-..261
FT                   2. pTZ19R EcoRI-HindIII 51bp 160..211
FT                   -> pAB366-2 4400bp [NsiI in 5' UTR am gene]"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-NsiI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT Aspergillus/Neurospora bleomycin resistance
FT                   gene (ble)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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