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ID   PAB366BEN  preliminary; circular DNA; SYN; 7200 BP.
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AC   M13630; ATCC77136;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Aspergillus/Neurospora/E.coli plasmid vector pAB366Ben - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAB366 or pAMPh366 from pRMH200 & pAm200
RC   pAB366-1 from pAB366
RC   pAB366-2 from pAB366 & pTZ19R
RC   pAB520 or pAmPh520 from pUC18 & pAB366-1
RC   pAB366Ben from pAB366 & pBT6
RA   Austin B., Hall R.M., Tyler B.M.;
RT   "Optimized vectors and selection for transformation of Neurospora
RT   crassa and Aspergillus nidulans to bleomycin and phleomycin
RT   resistance";
RL   Gene 93:157-162(1990).
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CC   Shuttle reporter plasmid to test expression of bleomycin resistance in
CC   transformants. [1]
CC   Expresses the mutant beta-tubulin gene conferring benomyl resistance.
CC   [1]
CC   A 2.5 kb SalI/HindIII fragment contains the complete tubulin gene and
CC   5' and 3' flanking regions. [2]
CC   The order of the major features in this plasmid is:pUC8 - EcoRI -
CC   BamHI - am promoter - ble - XhoI - am 3' region - tub-2 (benomyl
CC   resistance) - pUC8. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--5.1, 2.1; HindIII--4.0, 3.2; BamHI/HindIII--2.6, 2.5, 1.5,
CC   0.65; BamHI/XhoI--3.0, 1.35, 1.2, 0.9, 0.8; SalI/HindIII--3.3, 2.6,
CC   0.85, 0.55. (ATCC staff)
CC   A (GT)n repeat. (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAB366Ben)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Aspergillus nidulans)(Neurospora crassa)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 HindIII 2680bp 276..276
FT                   2. N. crassa HindIII-HindIII 3000bp, ben gene
FT                   -> pBT6 5680bp
FT                   1. pAB366 HindIII 4200bp, pUC8 261
FT                   2. pBT6 HindIII-HindIII 3000bp, ben gene
FT                   -> pAB366Ben 7200bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamNI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT Aspergillus/Neurospora bleomycin resistance
FT                   gene (ble)"
FT   CDS             0..0
FT                   /note="ANT Neurospora benomyl resistance gene (ben)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//