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ID   PAC1       preliminary; circular DNA; SYN; 10670 BP.
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AC   J01322; M10665; ATCC39532;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pAC1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAC1 from pBR322 & pDB248 & LEU2 gene & peno46, ENO1 gene
RA   ;
RT   ;
RL   Unpublished (1988).
RL   U.S. Patent No. 4,794,175 dated Dec. 27, 1988.
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RN   [2]
RC   from YEp13 & Aspergillus glucoamylase gene
RC   pAC1 from pBR322 & pDB248 & LEU2 gene & peno46, ENO1 gene
RC   pGC21 from pAC1 & Aspergillus glucoamylase gene
RC   pGAC9 from pAC1 & Aspergillus glucoamylase gene
RA   Innis M.A., Holland M.J., McCabe P.C., Cole G.E., Wittman V.P.,
RA   Tal R., Watt K.W.K., Gelfand D.H., Holland J.P., Meade J.H.;
RT   "Expression, glycosylation, and secretion of an Aspergillus
RT   glycoamylase by Saccharomyces cerevisiae";
RL   Science 228:21-26(1985).
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RN   [3]
RC   peno8, peno46 from pSF2124 & ENO1 gene
RA   Holland M.J., Holland J.P., Thill G.P., Jackson K.A.;
RT   "The primary structures of two yeast enolase genes. Homology
RT   between the 5' noncoding flanking regions of yeast enolase and
RT   glyceraldehyde-3-phosphate dehydrogenase genes";
RL   J. Biol. Chem. 256:1385-1395(1981).
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CC   Digests of the clone give the following sizes (kb):
CC   EcoRI--5.4, 3.8, 2.1; HindIII--11; PstI--11. (ATCC staff)
CC   Nucleotides 2282-2557: BamHI/SalI of pBR322 (375-650 of pBR322);
CC   2258-4773: XhoI/SalI of S. cerevisiae LEU2 from pCV13; 4774-8528:
CC   2 micron sequences from DB248; 8529-10672: Tth111I/EcoRI of pBR32
CC   (2218-4360 of pBR322). (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAC1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MM294)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. RSF2124 EcoRI 10900bp
FT                   dAT tailing
FT                   2. yeast, ENO1 promoter/gene/terminator
FT                   dAT tailing
FT                   -> peno46 12000bp
FT                   1. pBR322 EcoRI 4361bp 4360..4360
FT                   2. pDB248 EcoRI-SalI 3700bp, yeast 2 micron ori
FT                   3. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   4. peno46 HindIII-HindIII 14600bp, ENO1/pSF2124/ENO1
FT                   BAL31 nuclease:BAL31 nuclease
FT                   SalI linker 6bp gtcgac:SalI linker 6bp gtcgac
FT                   SalI-SalI 200bp, ENO1 promoter
FT                   :HindIII linker 6bp aagctt
FT                   5. peno46 HindIII-BglII 200bp, ENO1 terminator
FT                   -> pAC1 10670bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO E. coli enolase I gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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