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ID   PAC373     preliminary; circular DNA; SYN; 10000 BP.
AC   IG7005;
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pAC373 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-34
RC   pAc101, pAc311, pAc360 from AcMNPV
RC   pAc373 from pUC8 & linker & AcMNPV
RC   pAc373-CAT from pAc373
RC   pAc401, pAc409, pAc436, pAc461 from pAc311
RC   pAc461-RI-, pAc461-RI/PstI, pAc461-PstI from pAc461
RC   pAc510 from AcMNPV
RC   pAc610, pAc611 from pAc461
RC   pAc700, pAc701, pAc702 from pAc373
RC   pAcRP6, pAcRP18 from AcMNPV
RC   pAcYM1 from AcMNPV
RC   pEV55 from AcMNPV
RC   pVL77 from pUC19
RC   pVL101 from pAc401
RC   pVL106 from pAc436
RC   pAcC4, pAcC5 from AcMNPV
RA   Luckow V.A., Summers M.D.;
RT   "Trends in the development of baculovirus expression vectors";
RL   Biotechnology 6:47-55(1988).
CC   NM (pAC373)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(insect)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE ()
CC   PA (pUC8)(AcMNPV EcoRI-EcoRI 7000bp polyhedrin)
CC   BR ()
CC   OF (pAc373-CAT)(pAc700)(pAc701)(pAc702)(pVL77 from pAc373-CAT)
CC   OF (pVL101 from pAc401 and pVL77)(pVL106 from pAc436 and pVL77)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC8 HindIII-EcoRI 2635bp 261..2665..231
FT                   2. AcMNPV EcoRI-EcoRI 7000bp, polyhedrin gene
FT                   BAL31 nuclease
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   -> pAc373 9635bp"
FT   misc_binding    960..960
FT                   /note="SIT BstEII"
FT   misc_binding    1350..1350
FT                   /note="SIT PvuII"
FT   misc_binding    1900..1900
FT                   /note="SIT XhoI"
FT   misc_binding    2870..2870
FT                   /note="SIT SalI"
FT   misc_binding    3180..3180
FT                   /note="SIT SalI"
FT   misc_binding    3910..3910
FT                   /note="SIT EcoRV"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    4000..4000
FT                   /note="SIT BamHI"
FT   misc_binding    4080..4080
FT                   /note="SIT HindIII"
FT   misc_binding    4450..4450
FT                   /note="SIT KpnI"
FT   polyA_signal    4910..4910
FT                   /note="PLA AcMNPV polyhedrin gene"
FT   misc_binding    5030..5030
FT                   /note="SIT HindIII"
FT   misc_binding    6050..6050
FT                   /note="SIT SalI"
FT   misc_binding    6080..6080
FT                   /note="SIT HindIII"
FT   misc_binding    6500..6500
FT                   /note="SIT PvuII"
FT   misc_binding    6500..6500
FT                   /note="SIT PvuII"
FT   misc_binding    7100..7100
FT                   /note="SIT EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    9400..9400
FT                   /note="SIT HindIII"
SQ   Sequence 34 BP; 10 A; 10 C; 8 G; 6 T; 0 other;
     aaaaaaaccc gagatccgcg gatcctttcc tggg