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ID   PACAB3     preliminary; circular DNA; SYN; 10096 BP.
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AC   X70469;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pAcAB3 - incomplete, MCS.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-512
RC   plasmid from pAcUW3 & pAcYM1, AcNPV p10/ph promoter
RC   pAcAB4 from plasmid & pAcUW3 & linker
RC   pAcAB3 from pAcAB4
RC   pBacPAK6 from pBacPAK1
RC   from pBacPAK6
RA   Belyaev A.S., Roy P.;
RT   "Development of baculovirus triple and quadruple expression vectors:
RT   co-expression of three or four bluetongue virus proteins and the
RT   synthesis of bluetongue virus-like particles in insect";
RL   Nucleic Acids Res. 21:1219-1223(1993).
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RN   [2]
RP   1-512
RC   pAcAB3
RA   Belyaev A.S.;
RT   ;
RL   Submitted (08-FEB-1993) by:
RL   Belyaev A.S., NERC Inst of Virology & Environmental, Microbiology,
RL   Mansfield Rd, Oxford OX1 3SR, UK.
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CC   Promoter cassette of plasmid transfer vector pAcAB3 for insertion
CC   and expression of 3 foreign genes using the baculovirus expression
CC   system.
CC   MCS. oligonucleotide linker. plus not original sequence.
CC   NCBI gi: 287754
CC   NM (pAcAB3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAcUW3)(pAcYM1)
CC   BR (pAcAB4)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAcAB4 remove SmaI-BamHI 222bp, ph promoter
FT                   \ 10000bp
FT                   2. SmaI-BamHI linker 10bp cccgggatcc
FT                   -> pAcAB3 10096bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 512 BP; 191 A; 69 C; 79 G; 173 T; 0 other;
     ggatccccgg gatctgtgat tgtaaataaa atgtaattta cagtatagta ttttaattaa
     tatacaaatg atttgataat aattcttatt taactataat atattgtgtt gggttgaatt
     aaaggtcggg ccctttattc taggtttatt ggatcatgga gataattaaa atgataacca
     tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat
     aaatacggat ctctagaggc ctaataaact agagtcgagc aagaaaataa aacgccaaac
     gcgttggagt cttgtgtgct attttacaaa gattcagaaa tacgcatcac ttacaacaag
     ggggactatg aaattatgca tttgaggatg ccgggacctt taattcaacc caacacaata
     tattatagtt aaataagaat tattatcaaa tcatttgtat attaattaaa atactatact
     gtaaattaca ttttatttac aatcacagat ct
//