ID   PACAB4     preliminary; circular DNA; SYN; 10230 BP.
XX
AC   X70470;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pAcAB4 - incomplete, MCS.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-646
RC   plasmid from pAcUW3 & pAcYM1, AcNPV p10/ph promoter
RC   pAcAB4 from plasmid & pAcUW3 & linker
RC   pAcAB3 from pAcAB4
RC   pBacPAK6 from pBacPAK1
RC   from pBacPAK6
RA   Belyaev A.S., Roy P.;
RT   "Development of baculovirus triple and quadruple expression vectors:
RT   co-expression of three or four bluetongue virus proteins and the
RT   synthesis of bluetongue virus-like particles in insect";
RL   Nucleic Acids Res. 21:1219-1223(1993).
XX
RN   [2]
RP   1-646
RC   pAcAB4
RA   Belyaev A.S.;
RT   ;
RL   Submitted (08-FEB-1993) by:
RL   Belyaev A.S., NERC Inst of Virology & Environmental, Microbiology,
RL   Mansfield Rd, Oxford OX1 3SR, UK.
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CC   Promoter cassette of plasmid transfer vector pAcAB3 for insertion
CC   and expression of 4 foreign genes using the baculovirus expression
CC   system.
CC   MCS. oligonucleotide linker. plus not original sequence.
CC   NCBI gi: 287755
CC   NM (pAcAB4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAcUW3)(pAcYM1)
CC   BR (pAcAB4)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAcUW3 BglII-PpuMI 114bp, AcNPV p10 promoter
FT                   2. linker PpuMI-blunt end 7bp rggwccy
FT                   3. pAcYM1 EcoRV-BamHI 95bp, AcNPV ph promoter
FT                   blunt end:
FT                   -> fragment 216bp
FT                   1. fragment BglII-BamHI 216bp, AcNPV p10/ph promoters
FT                   2. pAcUW3 XbaI 10000bp
FT                   BamHI-XbaI-StuI-XbaI linker:XbaI-SpeI-SmaI-BamHI
FT                   \ linker
FT                   -> pAcAB4 10230bp [p10/ph//ph/p10]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 646 BP; 230 A; 88 C; 102 G; 226 T; 0 other;
     ggatccgtat ttataggttt ttttattaca aaactgttac gaaaacagta aaatacttat
     ttatttgcga gatggttatc attttaatta tctccatgat cagatccggg ggatcgatcc
     tctaggtttt attactagtc ccggggatct gtgattgtaa ataaaatgta atttacagta
     tagtatttta attaatatac aaatgatttg ataataattc ttatttaact ataatatatt
     gtgttgggtt gaattaaagg tcgggccctt tattctaggt tattggatca tggagataat
     taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta
     ataaaaaaac ctataaatac ggatctctag aggcctaata aactagagtc gagcaagaaa
     ataaaacgcc aaacgcgttg gagtcttgtg tgctatttta caaagattca gaaatacgca
     tcacttacaa caagggggac tatgaaatta tgcatttgag gatgccggga cctttaattc
     aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     taaaatacta tactgtaaat tacattttat ttacaatcac agatct
//