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ID   PACCL29    preliminary; circular DNA; SYN; 7800 BP.
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AC   IG5156;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pAcCL29 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAcCL29 from pAcYM1 & pUC118
RC   pAcCL29-1, pAcCL29-8 from pAcCL29 & pUC118
RA   Livingstone C., Jones I.;
RT   "Baculovirus expression vectors with single strand capability";
RL   Nucleic Acids Res. 17:2366-2366(1989).
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CC   MCS. not original sequence.
CC   NM (pAcCL29)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAcYM1)(pUC118)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAcYM1 XhoI-EcoRI 4700bp, expression/recomb
FT                   blunt end:blunt end
FT                   2. pUC118 remove HindIII-EcoRI 51bp 876..927, MCS
FT                   \ 3111bp
FT                   fill in:fill in
FT                   -> pAcCL29 7800bp [unique BamHI between polyhedrins]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 18 BP; 6 A; 3 C; 4 G; 5 T; 0 other;
     aaatacggat ccggttat
//