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ID   PACT1D     preliminary; circular DNA; SYN; 7400 BP.
AC   IG5138;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pAct1-D - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCOR101, pCOR102 from pBluescript II KS & pAct1-A
RC   pCOR103 from pBluescript II KS & pCOR101
RC   pCOR106 from pGEM-5Zf+ & pCOR101
RC   pCOR107 from pCOR106
RC   pCOR104 from pCOR101
RC   pCOR105 from pCOR104
RC   pCOR108 from pCOR102
RC   pCOR109 from pCOR102 & pGEM-5Zf+
RC   pCOR110, pCOR111 from pCOR109 & pBluescript II KS
RC   pCOR112 from pCOR111
RC   pCOR113 from pCOR111
RC   pCOR114 from pCOR110
RC   pCOR115, pCOR116 from pCOR109
RC   pCOR117 from pCOR116
RC   pAct1-F2 from pAct1-F & pCOR103
RC   pAct1-F3 from pAct1-F2
RC   pAct1-F4 from pAct1-F2 & pRAJ275
RC   pAct1-F5 from pAct1-F2 & pRAJ275
RC   pAct1-G72 from pAct1-F & pSP72
RC   pAct1-F6 from pAct1-G72
RC   pAct1-I72 from pAct1-F2 & pSP72
RC   pAct1-F7 from pAct1-I72 & pAct1-F2
RC   pAct1-R1 from pAct1-I72 & pAct1-R
RC   pBCG from pBluescript II KS & pBI221.1
RC   pBCG-A from pBCG & pAct1-F6
RC   pBCG-A4 from pBCG-A & pAct1-F4
RC   pBCG-A5 from pBCG-A & pAct1-F5
RC   pBCG-4I from pBCG & pAct1-F6
RC   pCI1GusN from pAI1GusN & pCaMVCN
RA   McElroy D., Blowers A.D., Jenes B., Wu R.;
RT   "Construction of expression vectors based on the rice actin 1 (Act1)
RT   5' region for use in monocot transformation";
RL   Mol. Gen. Genet. 231:150-160(1991).
RN   [2]
RC   pAct1-A from actin gene promoter
RC   pAct1-D from pAct1-A & nos gene
RC   pAct1-E from pAct1-A & nos gene
RC   pAct1-F, pAct1-G, pAct1-H from pAct1-D
RC   pAct1-J, pAct1-K, pAct1-L, pAct1-M, pAct1-R from pAct1-A
RC   pAct1-S, pAct1-T from pAct1-R
RA   McElroy D., Zhang W., Cao J., Wu R.;
RT   "Isolation of an efficient actin promoter for use in rice
RT   transformation";
RL   Plant Cell 2:163-171(1990).
RN   [3]
RC   pAI1GusN from pUC8 & GUS gene & ADH1 promoter
RA   Klein T.M., Gradzeil T., Fromm M.E., Sanford J.C.;
RT   "Factors influencing delivery into Zea mays cxells by
RT   high-velocity microprojectiles";
RL   Biotechnology 6:559-563(1988).
RN   [4]
RC   pCaMVneoAdh42, pCaMVneoAdh43 from pCaMVNEO
RC   pCaMVCN from CaMV 35S promoter & cat gene
RA   Callis J., Fromm M., Walbot V.;
RT   "Introns increase gene expression in cultured maize cells";
RL   Genes Dev. 1:1183-1200(1987).
RN   [5]
RC   pCaMVNEO from CaMV & neo gene
RA   Fromm M., Taylor L.P., Walbot V.;
RT   "Stable transformation of maize after gene transfer by
RT   electroporation";
RL   Nature 319:791-793(1986).
CC   GUS and Act1. new sequence?.
CC   NM (pAct1-D)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBluescript II KS+)(pGEM5Zf+)
CC   BR (pAct1-F)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBluescript II KS+
FT                   2. pGEM5Zf+
FT                   3. rice actin-1 gene
FT                   -> pAct1-A
FT                   1. pAct1-A, rice actin-1 gene
FT                   2. SmaI-XbaI, GUS-nos gene
FT                   -> pAct1-D 7400bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;