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ID   PACT2      preliminary; circular DNA; SYN; 7550 BP.
AC   ATCC87006;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid pACT2 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pACT, pACT2 from LEU2 gene
RA   Durfee T., Becherer K., Chen P.L., Yeh S.H., Yang Y., Kilburn A.E.,
RA   Lee W.H., Elledge S.J.;
RT   "The retinoblastoma protein associates with the protein
RT   phosphatase type 1 catalytic subunit";
RL   Genes Dev. 7:555-569(1993).
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--7.6; BamHI--7.6; BglII--7.5. (ATCC staff)
CC   Shuttle expression vector used to create fusion proteins consisting of
CC   the nuclear localization sequence from SV40 T antigen, the GAL4
CC   activation domain (aa 768-881) and a HA (hemagglutinin) epitope tag in
CC   frame with the activation domain. (personal communication)
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: Elledge S.J.
CC   NM (pACT2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)(expression)(shuttle - episomal)(DNA library)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp
FT                   2. SV40, T antigen
FT                   3. HA epitope
FT                   4. yeast, GAL4/LEU2
FT                   -> pACT2 7550bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   misc_binding    0..0
FT                   /note="MCS NdeI-NcoI-SfiI-SmaI-BamHI-EcoRI-XhoI-BglII"
FT   promoter        0..0
FT                   /note="PRO yeast ADH/ADC1"
FT   misc_feature    0..0
FT                   /note="GAL4 activation domain"
FT   misc_feature    0..0
FT                   /note="HA (hemagglutinin) epitope tag"
FT   terminator      0..0
FT                   /note="TER yeast ADC1"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;