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ID   PACYM1     preliminary; circular DNA; SYN; 103 BP.
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AC   D10356; D00126;
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DT   23-JUL-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Insect baculovirus vector pAcYM1 - incomplete, EcoRV-BamHI.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-103
RC   pAcEcoRII from pUC8 & AcNPV
RC   pAcRP6, pAcRP series from pAcEcoRII
RC   pAcRP61 from pAcRP6 & linker
RC   plasmid from pAcEcoRII & linker
RC   pAcYM1 from plasmid & pAcRP61
RA   Matsuura Y., Possee R.D., Overton H.A., Bishop D.H.;
RT   "Baculovirus expression vectors: the requirements for higb level
RT   expression of proteins, including glycoproteins";
RL   J. Gen. Virol. 68:1233-1250(1987).
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CC   Data have been obtained with recombinant baculoviruses which
CC   indicate that the level of expression of a foreign protein that
CC   replaces the AcNPV polyhedrin gene is related to the representation
CC   of the immediate 5' upstream sequences of that gene.
CC   NCBI gi: 220956
CC   NM (pAcYM1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (virus)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (Autographa californica nuclear polyhedrosis virus AcNPV)
CC   PA (pAcEcoRII)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 BamHI 2665bp 241..241
FT                   fill in
FT                   EcoRI linker 6bp gaattc
FT                   2. AcNPV EcoRI-EcoRI 7300bp, polyhedrin gene
FT                   -> pAcEcoRII 9971bp
FT                   1. pAcRP6 KpnI 9807bp, AcNPV
FT                   BAL31 nuclease
FT                   Klenow
FT                   BamHI linker 6bp ggatcc
FT                   remove small BamHI-BamHI, polyhedrin gene
FT                   -> pAcRP61 9800bp
FT                   1. pAcEcoRII largest BamHI-BamHI, AcNPV
FT                   BAL31 nuclease:BAL31 nuclease
FT                   Klenow:Klenow
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   large BamHI-BamHI
FT                   -> plasmid
FT                   1. plasmid small XhoI-BamHI
FT                   2. pAcRP61 large XhoI-BamHI
FT                   -> pAcYM1"
FT   misc_feature    1..88
FT                   /note="AcNPV polyhedrin gene leader sequence"
FT   misc_feature    41..41
FT                   /note="AcNPV polyhedrin mRNA start site"
FT   misc_binding    90..97
FT                   /note="SIT unique BamHI; linker"
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SQ   Sequence 103 BP; 41 A; 13 C; 14 G; 35 T; 0 other;
     tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa
     cagttttgta ataaaaaaac ctataaatac ggatccggtt att
//