Return to this vector's summary.
ID PACYM1 preliminary; circular DNA; SYN; 103 BP.
AC D10356; D00126;
DT 23-JUL-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE Insect baculovirus vector pAcYM1 - incomplete, EcoRV-BamHI.
KW cloning vector.
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
RC pAcEcoRII from pUC8 & AcNPV
RC pAcRP6, pAcRP series from pAcEcoRII
RC pAcRP61 from pAcRP6 & linker
RC plasmid from pAcEcoRII & linker
RC pAcYM1 from plasmid & pAcRP61
RA Matsuura Y., Possee R.D., Overton H.A., Bishop D.H.;
RT "Baculovirus expression vectors: the requirements for higb level
RT expression of proteins, including glycoproteins";
RL J. Gen. Virol. 68:1233-1250(1987).
CC Data have been obtained with recombinant baculoviruses which
CC indicate that the level of expression of a foreign protein that
CC replaces the AcNPV polyhedrin gene is related to the representation
CC of the immediate 5' upstream sequences of that gene.
CC NCBI gi: 220956
CC NM (pAcYM1)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (virus)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)(expression)
CC SE ()
CC PA (Autographa californica nuclear polyhedrosis virus AcNPV)
CC PA (pAcEcoRII)
CC BR ()
CC OF ()
CC OR ()
FH Key Location/Qualifiers
FT misc_feature 0..0
FT /note="1. pUC8 BamHI 2665bp 241..241
FT fill in
FT EcoRI linker 6bp gaattc
FT 2. AcNPV EcoRI-EcoRI 7300bp, polyhedrin gene
FT -> pAcEcoRII 9971bp
FT 1. pAcRP6 KpnI 9807bp, AcNPV
FT BAL31 nuclease
FT BamHI linker 6bp ggatcc
FT remove small BamHI-BamHI, polyhedrin gene
FT -> pAcRP61 9800bp
FT 1. pAcEcoRII largest BamHI-BamHI, AcNPV
FT BAL31 nuclease:BAL31 nuclease
FT BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT large BamHI-BamHI
FT -> plasmid
FT 1. plasmid small XhoI-BamHI
FT 2. pAcRP61 large XhoI-BamHI
FT -> pAcYM1"
FT misc_feature 1..88
FT /note="AcNPV polyhedrin gene leader sequence"
FT misc_feature 41..41
FT /note="AcNPV polyhedrin mRNA start site"
FT misc_binding 90..97
FT /note="SIT unique BamHI; linker"
SQ Sequence 103 BP; 41 A; 13 C; 14 G; 35 T; 0 other;
tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa
cagttttgta ataaaaaaac ctataaatac ggatccggtt att