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ID   PAD22      preliminary; circular DNA; SYN; 23000 BP.
AC   ATCC37321;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli cosmid vector pAD22 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCVD300 from pRK290 & pREG153
RC   pCVD301 from M13mp7 & pCVD300
RC   pAD22 from pCVD301 & YRp7
RA   Datta A.R., Kaper J.B., MacQuillan A.M.;
RT   "Shuttle cloning vectors for the marine bacterium Vibrio
RT   parahaemolyticus";
RL   J. Bacteriol. 160:808-811(1984).
CC   Broad host range cosmid vector which can be selected and replicate in
CC   E.coli, Saccharomyces cerevisiae, and Vibrio parahaemolyticus.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pAD22)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli JA300)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp
FT                   2. phage P1
FT                   -> pREG153
FT                   1. pRK290
FT                   2. pREG153
FT                   -> pCVD300 17000bp
FT                   1. M13mp7, ori
FT                   2. pCVD300 17000bp
FT                   -> pCVD301 17000bp
FT                   1. pCVD301 EcoRI 17000bp, 3' MCS
FT                   alkaline phosphatase
FT                   2. YRp7 EcoRI 5817bp 2..2, TRP1/ARS/ori
FT                   -> pAD22 23000bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BamHI-SalI-PstI-SalI-BamHI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI-SalI-PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;