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ID   PAHF3      preliminary; circular DNA; SYN; 53 BP.
AC   D10362; D00192;
DT   23-JUL-1992 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Saccharomyces plasmid vector pAHF3 - incomplete, ADH pro/P-450 junct.
KW   cloning vector; ADH1; cytochrome P-450.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-53
RC   pHP2, pHP2-1, pHP3 from pBR322 & pcP-450pb4
RC   pAHF3 from pHP3 & pAAH5
RC   pAHFdeltaN3 from pHP3 & pAAH5
RA   Imai Y.;
RT   "Cytochrome P-450 related P-450-4 from phenobarbital-treated rabbit
RT   liver: Molecular cloning of cDNA and characterization of cytochrome
RT   P-450 obtained by its expression in yeast cells";
RL   J. Biochem. 101:1129-1139(1987).
RN   [2]
RC   pcP-450pb1, pcP-450pb2, pcP-450pb4 from rat cytochrome P-450
RA   Fujii-Kuriyama Y., Mizukami Y., Kawajiri K., Sogawa K., Muramatsu M.;
RT   "Primary structure of a cytochrome P-450: coding nucleotide
RT   sequence of phenobarbital-inducible cytochrome P-450 cDNA from
RT   rat liver";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:2793-2797(1982).
RN   [3]
RC   pAH5, pAH9, pAH10, pAH21 from YEp13 & ADC1 promoter
RC   pAAH5 from YEp13 & ADC1 promoter/ADH terminator
RC   pAAR6 from YRp7 & yeast 2 micron & ADC1 promoter/ADH terminator
RC   pMA56 from YRp7 & yeast 2 micron & ADC1 promoter
RC   pABade8 from YEp13 & ADC1 promoter & Drosophila ADE8 gene
RC   pAXalpha11 from YEp13 & ADC1 promoter & MATalpha1 gene
RA   Ammerer G.;
RT   "Expression of genes in yeast using the ADC1 promoter";
RL   Meth. Enzymol. 101:192-201(1983).
CC   clone pHP3 = rabbit minor cytochrome P-450
CC   pAHF3 contained the entire coding sequence of pHP3 (RABP45PH3), but
CC   nucleotide sequences for the N-terminal region of pHP3 protein
CC   (from the 2nd to the 23rd amino acid) were deleted in pAH-delta-N3.
CC   On introduction into yeast AH22 cells, the absorption spectrum of
CC   cytochrome P-450 was detected in the microsomal fraction from the
CC   transformed cells csrrying pAHF3. On the other hand, cytochrome
CC   P-450 could not be detected spectrophotometrically in any
CC   subcellular fractions from the yeast cells carrying pAH-delta-N3,
CC   although the transcript of pHP3 insert was detected in RNA blot
CC   analysis.
CC   NCBI gi: 220960
CC   NM (pAHF3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (yeast AH22)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAAH5)(pHP3)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. YEp13 remove HindIII-HindIII, 3' yeast ori
FT                   BamHI
FT                   2. yeast BamHI-HindIII 1500bp, ADC1 promoter
FT                   3. yeast HindIII-BamHI 450bp, ADC1 terminator
FT                   -> pAAH5 12500bp
FT                   1. pBR322
FT                   2. rat P-450 gene
FT                   -> pcP-450pb4
FT                   1. pBR322 PstI
FT                   dG tailing
FT                   2. pcP-450pb4, rat P-450 gene
FT                   dC tailing
FT                   -> pHP3
FT                   1. pHP3 BamHI-BamHI 1629bp
FT                   Klenow fill in:Klenow fill in
FT                   ClaI linker 8bp:ClaI linker 8bp
FT                   Klenow fill in:Klenow fill in
FT                   HindIII linker:HindIII linker
FT                   HindIII-HindIII
FT                   2. pAAH5 HindIII, between ADH1 prom and term
FT                   -> pAHF3"
FT   CDS             36..>53
FT                   /note="GEN rabbit minor cytochrome P-450 polypeptide;
FT                   BamHI fragment of clone pHP3"
SQ   Sequence 53 BP; 15 A; 13 C; 13 G; 12 T; 0 other;
     agctatacca agcatacaat caactcaagc ttgcgatgga tcccgtggtg gtg