back Return to this vector's summary.
ID   PAJ3148    preliminary; circular DNA; SYN; 9800 BP.
XX
AC   ATCC39137;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/Brevibacterium/Corynebacterium plasmid vector pAJ3148 - inco.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pHM1519 from Corynebacterium glutamicum
RC   pAJ440 from pHM1519
RC   pAJ655 from pHM1519
RC   pAJ1844 from pHM1519
RC   pAJ3148 from pUB110 & pHM1519
RA   ;
RT   ;
RL   Unpublished (1985).
RL   U.S. Patent No. 4,514,502 dated Apr. 30, 1985.
XX
RN   [2]
RC   pAL286 from Corynebacterium glutamicum
RA   Miwa K., Terabe M., Ito K., Ishida M., Matsui H., Nakamori S.,
RA   Sano K.;
RT   "Composite plasmid";
RL   Unpublished (1983).
RL   European Patent Appl. 0093611 dated Nov. 9, 1983.
XX
CC   pUB110 was cut with BamHI and pHM1519 (a natural isolate from
CC   Corynebacterium glutamicum) was cut with BglII. The construct has 1
CC   copy of pUB110 and two copies of pHM1519.
CC   This vector replicates in Corynebacterium glutamicum and Bacillus
CC   subtilis.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pAJ3148)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Corynebacterium glutamicum [ATCC 13032])
CC   HO (Corynebacterium glutamicum)(Bacillus subtilis)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Corynebacterium glutamicum
FT                   -> pHM1519 4300bp
FT                   1. pHM1519 4300bp, ori
FT                   2. pUB110 4548bp, ori/kan gene
FT                   -> pAJ3148 9800bp"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   rep_origin      0..0
FT                   /note="ORI Corynebacterium pHM1519"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//