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ID   PAJ440     preliminary; circular DNA; SYN; 9000 BP.
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AC   ATCC39139;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Brevibacterium/Corynebacterium plasmid vector pAJ440 - incom.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHM1519 from Corynebacterium glutamicum
RC   pAJ440 from pHM1519
RC   pAJ655 from pHM1519
RC   pAJ1844 from pHM1519
RC   pAJ3148 from pUB110 & pHM1519
RA   ;
RT   ;
RL   Unpublished (1985).
RL   U.S. Patent No. 4,514,502 dated Apr. 30, 1985.
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CC   Restriction digests of the clone give the following sizes (kb):
CC   BglII--9.0. (ATCC staff)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pAJ440)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Bacillus subtilis RM125)(Bacillus subtilis)
CC   HO (Brevibacterium)(Corynebacterium glutamicum)
CC   HO (Brevibacterium lactofermentum [ATCC 39134])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Corynebacterium glutamicum
FT                   -> pHM1519 4500bp
FT                   1. pHM1519 4500bp, ori
FT                   2. pUB110 4548bp, ori/kan gene
FT                   -> pAJ440 9000bp"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   rep_origin      0..0
FT                   /note="ORI Corynebacterium pAM330"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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