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ID   PAJC264    preliminary; circular DNA; SYN; 12 BP.
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AC   IG5095;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pAJC264 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAC1 from ptac12 & lamB gene & lacIq gene
RC   pAJC264 from oligo & pAC1
RA   Charbit A., Molla A., Saurin W., Hofnung M.;
RT   "Versatility of a vector for expressing foreign polypeptides at the
RT   surface of gram-negative bacteria";
RL   Gene 70:181-189(1988).
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CC   MCS. oligonucleotide linker.
CC   NM (pAJC264)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAC1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ptac12
FT                   2. lamB gene
FT                   3. lacIq gene
FT                   -> pAC1 [tac]
FT                   1. pAC1, between AA153 and AA154 in lamB gene
FT                   2. BamHI linker 12bp cgggatcccgcc
FT                   -> pAJC264"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 12 BP; 1 A; 6 C; 4 G; 1 T; 0 other;
     cgggatcccg cc
//