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ID   PAK1003    preliminary; circular DNA; SYN; 7100 BP.
AC   ATCC37425;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Higher plants/E.coli plasmid vector pAK1003 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pAR1200 from pUC8 & pTiAch5
RC   pOP1 from pAR1200 & pBR322
RC   pOP443 from pOP1 & pUC8
RC   pOP4434 from pOP443
RC   pOP4439 from pOP443
RC   pOP44392 from pOP4439 & pBR322
RC   pAK1003 from pOP4434 & pLGV1103
RC   pSL3 from pBR325
RC   pAK1242 from pAK1003 & pSL3
RC   pAK2004 from pOP44392 & pLGV1103
RA   Velten J., Velten L., Hain R., Schell J.;
RT   "Isolation of a dual plant promoter fragment from the Ti plasmid of
RT   Agrobacterium tumefaciens";
RL   EMBO J. 3:2723-2730(1984).
RN   [2]
RC   pMH1 from pLGV2382 & pSV0cat
RC   pMH2 from pMH1 & pKC7, pea ssu gene
RC   pLGVTiSS-CAT from pMH2 & pTiC58
RA   Herrera-Estrella L., Van Den Broeck G., Maenhaut R., Van Montagu M.,
RA   Schell J., Timko M., Cashmore A.;
RT   "Light-inducible and chloroplast-associated expression of a
RT   chimaeric gene introduced into Nicotiana tabacum using a Ti
RT   plasmid vector";
RL   Nature 310:115-120(1984).
CC   Selection-expression plasmid for use in transformation of higher
CC   plants.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAK1003)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(higher plants)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Agrobacterium tumefaciens, crown gall
FT                   -> pTiAch5
FT                   1. pUC8 SmaI 2665bp 238..238
FT                   2. pTiAch5 AluI-AluI 676bp, 1'-2' intergenic region
FT                   -> pAR1200 [unique ClaI at init codon of 2' ORF]
FT                   1. pAR1200 ClaI-HindIII, 1' to MCS pUC8 261
FT                   2. pBR322 remove ClaI-HindIII 5bp 25..30, 4356bp
FT                   -> pOP1
FT                   1. pOP1 HindIII, MCS pUC8 261/pBR322 tet
FT                   BAL31 nuclease
FT                   BamHI, pBR322 tet 376
FT                   Klenow
FT                   -> plasmid [BamHI]
FT                   1. plasmid EcoRI-BamHI, pBR322 to Agrobacterium
FT                   2. pUC8 remove EcoRI-BamHI 10bp 231..241, MCS 2655bp
FT                   -> pOP443 [BamHI 3bp 5' to start codon]
FT                   1. pOP443 remove EcoRI-ClaI, 2' promoter
FT                   Klenow
FT                   -> pOP4434 [EcoRI at 2' start codon site]
FT                   1. AcMNPV, NOS promoter
FT                   2. Tn1831, strep/spec genes/#?
FT                   -> pLGV1103
FT                   1. pOP4434 EcoRI-BamHI, 1'-2' promoter
FT                   2. pLGV1103 remove EcoRI-BclI, NOS promoter
FT                   -> pAK1003 7100bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO A. tumefaciens T-DNA gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
SQ   Sequence 29 BP; 11 A; 7 C; 5 G; 6 T; 0 other;
     cgatacacca aatcgaattc ttgaagacg