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ID   PAL146     preliminary; circular DNA; SYN; 146 BP.
AC   M10045;
DT   20-MAY-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pAL146 - incomplete, 146 bp palindrome.
KW   cloning vector; palindrome.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-146
RC   pGJ68 from pAT153 & pGJ53, kan gene
RC   pGJ71 from pGJ67 & pAT153
RC   pGJ87 from P. syringae
RC   pGJ106 from pGJ68 & pKT210
RC   pGJ128 from pBR322 & pGJ68 & pGJ87
RC   pBR322-PAL147 from pBR322 & pSVoriH
RC   pGJ68-PAL147 from pGJ68 & pSVoriH
RC   pGJ106-PAL147 from pGJ106 & pSVoriH
RC   pGJ128-PAL147 from pGJ128 & pSVoriH
RC   pGJ106-2xPAL147 from pGJ106-PAL147 & pSVoriH
RC   pAL146 or pBR322-PAL146 from pBR322 & piVX
RC   pRK404-PAL146 from pBR322-PAL146 & pRK404
RC   pGJ128-PAL146 from pBR322-PAL146 & pGJ128
RC   pBR322-PAL114 from pBR322-PAL146
RA   Warren G.J., Green R.L.;
RT   "Comparison of physical and genetic properties of palindromic DNA
RT   sequences";
RL   J. Bacteriol. 161:1103-1111(1985).
RN   [2]
RP   1-146
RC   pAL146
RA   Warren G.J.;
RT   ;
RL   Submitted (14-AUG-1985) by:
RL   Warren G.J., .
RN   [3]
RC   pGJ53 from pAT273 & Tn5, kan gene
RA   Hagan C.E., Warren G.J.;
RT   "Lethality of palindromic DNA and its use in selection of
RT   recombinant plasmids";
RL   Gene 19:147-151(1982).
RN   [4]
RC   pCEH1 from RSF1050 & Tn5, kan gene
RC   pCEH15 from pACYC184 & Tn5, kan gene
RC   pGJ67 from pACYC184 & pGJ53
RA   Hagan C.E., Warren G.J.;
RT   "Viability of palindromic DNA is restored by deletions occurring
RT   at low but variable frequency in plasmids of E. coli";
RL   Gene 24:317-326(1983).
RN   [5]
RC   pSVoriH, pSVori2172, pSVori1x21 from pMK2004 & SV40 ori
RA   Bergsma D.J., Olive D.M., Hartzell S.W., Byrne B.J., Subramanian K.N.;
RT   "Cyclization of linear chimeric plasmids in vivo by a novel
RT   end-to-end joining reaction or by intramolecular recombination:
RT   one of the products contains a 147-bp perfect pailindrome stable
RT   in E. coli";
RL   Gene 20:157-167(1982).
CC   The presence of the palindrome apparently inhibited the spontaneous
CC   multimerization of plasmids that occur in Rec+ strains of E.coli.
CC   NCBI gi: 207909
CC   NM (pAL146)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli K-12)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (E.coli K-12)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. piVX BamHI-ClaI 70bp, MCS
FT                   3. piVX ClaI-BamHI 70bp, MCS
FT                   -> pAL146"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 146 BP; 35 A; 38 C; 38 G; 35 T; 0 other;
     ggatccgggc aacgttgttg ccattgctgc aggcggagaa ctggtaggta tggaagatct
     ctagaagctt atcgataagc ttctagagat cttccatacc taccagttct ccgcctgcag
     caatggcaac aacgttgccc ggatcc