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ID   PAL4       preliminary; circular DNA; SYN; 7000 BP.
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AC   ATCC37465;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pAL4 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAL49 from pKO-1 & pML21 & E.coli chromosome
RC   pAL43 from pKO-1 & pML21 & E.coli chromosome
RC   pAL4 from pML21 & E.coli chromosome
RC   from pBR322
RA   Leonard A.C., Helmstetter C.E.;
RT   "Cell cycle-specific replication of Escherichia coli
RT   minichromosomes";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:5101-5105(1986).
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RN   [2]
RC   pAL20, pAL22, pAL220 from pAL1
RC   pAL4, pAL5, pAL6 from pAL1
RA   Leonard A.C., Whitford W.G., Helmstetter C.E.;
RT   "Involvement of DNA superhelicity in minichromosome maintenance in
RT   Escherichia coli";
RL   J. Bacteriol. 161:687-695(1985).
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RN   [3]
RC   pAL1 from pML21 & E.coli oriC
RC   pAL2 from pAL1
RC   from pAL2
RA   Leonard A.C., Hucul J.A., Helmstetter C.E.;
RT   "Kinetics of minichromosome replication in Escherichia coli";
RL   J. Bacteriol. 149:499-507(1982).
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RN   [4]
RC   pML21 from pVH51 & pSC105, kan gene
RC   pML31 from F' lac & pML21, kan gene
RA   Lovett M.A., Helinski D.R.;
RT   "Method for the isolation of the replication region of a
RT   bacterial replicon: construction of a mini-F'Km plasmid";
RL   J. Bacteriol. 127:982-987(1976).
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RN   [5]
RC   pAL1 from pML21 & E.coli oriC
RC   pAL2 from pAL1
RA   Leonard A.C., Weinberger M., Munson B.R., Helmstetter C.E.;
RT   "The effects of oriC-containing plasmids on host cell growth";
RL   Mechanistic Studies of DNA Rep and Genetic Recomb 0:171-179(1980).
RL   Alberts B., ed., Academic Press, New York.
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CC   The plasmid minichromosome exhibits a moderate degree of instability
CC   and should be propagated in recA- strains grown in at least 50ug/ml
CC   kanamycin.
CC   A plasmid which replicates from the resident chromosome origin of
CC   replication, oriC.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pAL4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli B/r F26-44)(E.coli)(E.coli recA- strains)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   -> pSC105 16163bp
FT                   1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   2. pVH51 EcoRI 3847bp 3846..3846
FT                   -> pML21 10747bp
FT                   1. pML21 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   2. E.coli EcoRI-EcoRI 9700bp, oriC
FT                   -> pAL1 16600bp [kan gene]
FT                   1. pAL1 remove PstI-PstI 9600bp
FT                   -> pAL4 7000bp [oriC/Tn903 kan]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-PstI-KpnI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli oriC"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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