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ID   PAL7       preliminary; circular DNA; SYN; 6500 BP.
AC   ATCC37797;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Schizosaccharomyces/E.coli plasmid vector pAL7 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pAU5 from pBR322 & pFL20
RC   pAU9 from pBR322 & pFL20
RC   pAU21 from pBR322 & pFL20
RC   pAL7 from pBR322 & pDB262
RC   pAL19 from pBR322 & pDB262
RC   pEL11 from pAL7 & yeast 2 micron
RA   Okazaki K., Okazaki N., Kume K., Jinno S., Tanaka K., Okayama H.;
RT   "High-frequency transformation method and library transducing
RT   vectors for cloning mammalian cDNAs by trans-complementation of
RT   Schizosaccharomyces pombe";
RL   Nucleic Acids Res. 18:6485-6489(1990).
RN   [2]
RC   pFL20 from pBR322-8 & S. pombe DNA
RC   pFL21 from pBR322-8 & S. pombe PPR1 gene
RC   from pJDB207 & pFL20
RC   from pJDB207 & pFL21
RC   pFL11 from pFL20 & PPR1-1 gene
RC   pFL12 from pFL20 & PPR1-2 gene
RA   Losson R., Lacroute F.;
RT   "Plasmids carrying the yeast OMP decarboxylase structural and
RT   regulatory genes: transcription regulation in a foreign
RT   environment";
RL   Cell 32:371-377(1983).
RN   [3]
RC   pHB12 from pJDB207 & S.cerevisiae PPR1 gene
RA   Losson R., Lacroute F.;
RT   "Cloning of a eukaryotic regulatory gene";
RL   Mol. Gen. Genet. 184:394-399(1982).
CC   Plasmid linearized with PstI can be used to introduce cDNA libraries
CC   constructed in pcD or pcD2 vectors into Schizosaccharomyces pombe,
CC   resulting in the formation, by homologous recombination, of
CC   concatamers maintained as episomal DNA.
CC   Contains the ars and stb sequences from Schizosaccharomyces pombe and
CC   the LEU2 gene from Saccharomyces cerevisiae.  The PstI site interrupts
CC   the beta-lactamase gene.
CC   Shares pBR322 sequences from the PvuII site to the EcoRI site with
CC   pcD.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--3.3, 1.3, 1.2, 0.9; HindIII--6.6; PstI--6.6. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAL7)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Schizosaccharomyces pombe)(E.coli)
CC   HO (Schizosaccharomyces pombe [ATCC 38399])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pML2 2900bp
FT                   -> pCR11 2900bp
FT                   1. pCR11 2900bp
FT                   2. lambda, cI gene
FT                   -> pKB155
FT                   1. pKB155
FT                   2. pMB9
FT                   -> pKB158
FT                   1. pBR322 remove PstI-EcoRI 748bp 3612..4360, 3613bp
FT                   2. pKB158 EcoRI-PstI 1600bp,
FT                   \ lambda cI gene/oR/pRM/cro gene
FT                   -> pSLS102 5200bp
FT                   1. pMB9 5300bp
FT                   2. E. coli, lac operator
FT                   -> pMB9-lac21 5500bp
FT                   1. pSLS102 remove HindIII-BglII/BamHI 346bp 30..376,
FT                   \ tet prom/4900bp
FT                   E. coli DNA polymerase:E. coli DNA polymerase
FT                   2. pMB9-lac21 EcoRI-EcoRI 29bp, lac operator
FT                   E. coli DNA polymerase:E. coli DNA polymerase
FT                   -> pTR262 4900bp
FT                   1. pTR262
FT                   2. pDB248, yeast ARS/LEU2 gene
FT                   -> pDB262 10600bp
FT                   1. pBR322 PvuII-EcoRI 2293bp 2067..4360, ori/amp gene
FT                   2. pDB262 HincII-HincII, ARS/stb/LEU2 gene
FT                   -> pAL7 6500bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;