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ID   PALCA1S    preliminary; circular DNA; SYN; 4500 BP.
AC   ATCC53368;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Yeast/E. coli plasmid pALCA1S - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pALCA1S from pDG6
RA   ;
RT   ;
RL   Unpublished (1993).
RL   U.S. Patent No. 5,198,345 dated March 30, 1993.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--4.5; BamHI--4.5; HindIII--4.5. (ATCC staff)
CC   Constructed from pDG6 (ATCC 53169) by removing the endoglucanase
CC   coding region and the initiator codon of the alcA, retaining the TATA
CC   box and the transcription start site.A synthetic signal peptide coding
CC   region was inserted. [1]
CC   Note: This material is cited in a U.S and/or other Patent and may not
CC   be used to infringe the patent claims.
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)(Gist-Brocades N.V.)
CC   HO (E.coli)(E.coli JM83)(filamentous fungi)
CC   CP ()
CC   FN (cloning)(expression)(secretion)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pDG6 remove, endoglucanase gene/alcA init
FT                   2. oligo, signal peptide region
FT                   -> pALCA1S [TATA/transcription start site]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast ARGB gene"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-...-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   promoter        0..0
FT                   /note="PRO E. coli alcA"
FT   misc_feature    0..0
FT                   /note="signal peptide"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;