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ID   PALPHA3    preliminary; circular DNA; SYN; 7600 BP.
AC   ATCC53220;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector palpha3 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   palpha3 from yeast 2 micron & alpha factor gene & pBR322 & TRP1 gene
RA   ;
RT   ;
RL   Unpublished (1989).
RL   U.S. Patent No. 4,882,282 dated Nov. 21, 1989.
CC   The EcoRI/KpnI promoter cassette contains the pre-pro alpha factor
CC   promoter and leader plus ATG.
CC   Restriction digests of the clone give the following sizes (kb):
CC   KpnI--7.6; PstI--3.3, 1.8, 1.6, 0.8; EcoRI--7.6; HindIII--5.4, 2.0;
CC   BamHI--uncut. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (palpha3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli RR1)(Saccharomyces cerevisiae X2181-1B)(E.coli)
CC   HO (Saccharomyces cerevisiae 79)(Saccharomyces cerevisiae DBY746)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast 2 micron, ori
FT                   3. yeast, alpha factor gene
FT                   4. yeast, TRP1 gene
FT                   -> palpha3 7600bp"
FT   misc_binding    0..0
FT                   /note="MCS KpnI-PstI-AvrII-NcoI-KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-PstI-AvrII-NcoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO yeast pre-pro alpha factor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;