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ID   PALS1      preliminary; circular DNA; SYN; 13100 BP.
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AC   M30889;
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DT   15-JUN-1990 (Rel. 5, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Neurospora plasmid vector pALS-1 - incomplete, ARS.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-316
RC   pALS-1-1, pALS-1-2 from pALS-1
RC   pALS-1-(MC) from pALS-1-1
RA   Stohl L.L., Akins R.A., Lambowitz A.M.;
RT   "Characterization of deletion derivatives of an autonomously
RT   replicating Neurospora plasmid";
RL   Nucleic Acids Res. 12:6169-6178(1984).
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RN   [2]
RC   pLSHQ2 from pBR325 & pRC57, Neurospora ga-2 gene
RC   pLSP-1 from pBR322 & N. intermedia P405-Labelle 4.1 kb plasmid
RC   pALS-1 from pLSHQ2 & pLSP-1
RC   pALS-1-1, pALS-2 from pALS-1
RC   pALS-1-2, pALS-1-3 from pALS-1-1
RC   pALS-2-2 from pALS-2
RA   Stohl L.L., Lambowitz A.M.;
RT   "Characterization of deletion derivatives of an autonomously
RT   replicating Neurospora plasmid";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:1058-1062(1983).
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RN   [3]
RC   pRC57 from pBR322 & Neurospora ga-2 gene
RA   Case M.;
RT   ;
RL   Unpublished (1983).
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CC   NCBI gi: 208948
CC   NM (pALS-1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII
FT                   2. N. crassa HindIII-HindIII, qa-2 gene
FT                   -> pRC57
FT                   1. pBR325 HindIII
FT                   2. pRC57 HindIII-HindIII 3000bp, Neurospora ga-2 gene
FT                   -> pLSHQ2
FT                   1. pBR322 PstI
FT                   2. N. intermedia P405-Labelle PstI, 4100bp plasmid
FT                   -> pLSP-1
FT                   1. pLSHQ2 PstI
FT                   2. pLSP-1 PstI-PstI, 4100bp plasmid
FT                   -> pALS-1 13100bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 316 BP; 98 A; 41 C; 75 G; 98 T; 4 other;
     gtttgcgcaa cgttgttgcc attgctgcag cgtaaaagac gttctcatcc aaagaaaagt
     ttttatgatc ttatgtataa gttgattggt aattctatat acggtttagc ttctcagggt
     attggtggta aaaagacttt tgatataaaa accaaaannn ngggtgtgag tattgagttc
     gtagcctatg ggttgtgaaa tgactgttaa gagtggggtg atggtaccgt ttgtcaaaag
     atctaaaaga attgaaaaag atcgtctgga ttataaaagt ccctatagac aaataatgtc
     agatctgcag gcatcg
//