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ID   PALS2      preliminary; circular DNA; SYN; 9000 BP.
XX
AC   M30888;
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DT   15-JUN-1990 (Rel. 5, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Neurospora plasmid vector pALS2 - incomplete, ARS.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-70
RC   pALS-1-1, pALS-1-2 from pALS-1
RC   pALS-1-(MC) from pALS-1-1
RA   Stohl L.L., Akins R.A., Lambowitz A.M.;
RT   "Characterization of deletion derivatives of an autonomously
RT   replicating Neurospora plasmid";
RL   Nucleic Acids Res. 12:6169-6178(1984).
XX
RN   [2]
RC   pLSHQ2 from pBR325 & pRC57, Neurospora ga-2 gene
RC   pLSP-1 from pBR322 & N. intermedia P405-Labelle 4.1 kb plasmid
RC   pALS-1 from pLSHQ2 & pLSP-1
RC   pALS-1-1, pALS-2 from pALS-1
RC   pALS-1-2, pALS-1-3 from pALS-1-1
RC   pALS-2-2 from pALS-2
RA   Stohl L.L., Lambowitz A.M.;
RT   "Characterization of deletion derivatives of an autonomously
RT   replicating Neurospora plasmid";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:1058-1062(1983).
XX
CC   NCBI gi: 208949
CC   NM (pALS2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pALS-1 remove PstI-PstI, 4100bp plasmid
FT                   -> pALS-2 [no PstI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 70 BP; 8 A; 16 C; 21 G; 25 T; 0 other;
     gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     tggcttcatt
//