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ID   PAM3       preliminary; circular DNA; SYN; 716 BP.
XX
AC   X69575;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Mycobacterium plasmid vector pAM3 - incomplete.
XX
KW   cloning vector.
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OS   Mycobacterium paratuberculosis
OC   Unclassified.
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RN   [1]
RC   pAM3 from pGEM-2 & lambda gt11 & M. paratuberculosis genome
RC   pAM310 from pAM3 & pNM482
RC   pAM320 from pAM310 & pRR3
RC   pWR30 from pSL180
RC   pWR31 from pAM3 & pJN1
RC   pWR32, pWR33 from pWR31 & pRR3
RC   pAM311 from pUC18 & pAM3
RC   pAM312 from pUC18 & pAM3
RC   pTG959 from pUC18 & lambda, pL
RC   pJN1 from pNM482 & pTG959
RA   Murray A., Winter N., Lagranderie M., Hill D.F., Rauzier J., Timm J.,
RA   Leclerc C., Moriarty K.M., Gheorghiu M., Gicquel B.;
RT   "Expression of Escherichia coli beta-galactosidase in Mycobacterium
RT   bovis BCG using an expression system isolated from Mycobacterium
RT   paratuberculosis which induced humoral and cellular";
RL   Mol. Microbiol. 6:3331-3342(1992).
XX
RN   [2]
RC   pMB15, pMB22, pMB115 from M. paratuberculosis IS900
RA   Green E.P., Tizard M.L., Moss M.T., Thompson J., Winterbourne D.J.,
RA   McFadden J.J., Hermon-Taylor J.;
RT   "Sequence and characteristics of IS900, an insertion element
RT   identified in a human Crohn's disease isolate of Mycobacterium
RT   paratuberculosis";
RL   Nucleic Acids Res. 17:9063-9073(1989).
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RN   [3]
RC   pAL5000 from Mycobacterium fortuitum
RC   pBTZ from pTZ19R & pAL5000
RC   pAL817 from pTZ19R & pAL5000
RC   pB4 from pBTZ & Tn903, kan gene
RC   pAL8 from pAL817 & Tn903, kan gene
RC   pALd1 from pAL8
RC   pRRb from pUC19 & Tn903, kan gene
RC   pRR3 from pRRb & pAL5000
RA   Ranes M.G., Rauzier J., Lagranderie M., Gheorghiu M., Gicquel B.;
RT   "Functional analysis of pAL5000, a plasmid from Mycobacterium
RT   fortuitum: construction of a mini Mycobacterium-Escherichia coli
RT   shuttle vector";
RL   J. Bacteriol. 172:2793-2797(1990).
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CC   Mycobacterium paratuberculosis IS900 insertion element.
CC   ORF2 is located in IS900 on the complementary strand to the gene
CC   coding for transposase.
CC   NCBI gi: 395189
CC   NM (pAM3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Mycobacterium)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Mycobacterium paratuberculosis)(pGEM-2)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M. paratuberculosis 3800bp
FT                   recombination
FT                   2. lambda gt11
FT                   -> phage
FT                   1. pGEM-2 remove EcoRI-BamHI, MCS
FT                   2. phage EcoRI-BamHI 1600bp, M. paratuber... pAN/ORF2
FT                   -> pAM3"
FT   rep_origin      0..0
FT                   /note="ORI Mycobacterium"
FT   -35_signal      83..88
FT                   /note="S35 Mycobacterium ORF2"
FT   -10_signal      106..111
FT                   /note="S10 Mycobacterium ORF2"
FT   prim_transcript 118..>716
FT                   /note="RNA Mycobacterium ORF2 mRNA"
FT   RBS             149..154
FT                   /note="RBS Mycobacterium ORF2"
FT   CDS             160..>716
FT                   /note="GEN Mycobacterium ORF2; partial;
FT                   NCBI gi: 395190;
FT                   original sequence comes from Fig. 2 [1]"
XX
SQ   Sequence 716 BP; 112 A; 204 C; 277 G; 123 T; 0 other;
     gatcccgtga caagcccgaa gagcccgcga ccgtgcggtc gtcgacgacc gagtgtgagc
     agaccccctg gtgaagggtg aatcgacagg tacacacagc cgccatacac ttcgcttcat
     gcccttacgg ggggcggcca acccagaagg agattctcaa tgacgttgtc aagccgccgc
     ggtagtggtt gcggggtggt agacagcgtg gtcgcgcagc atggcccaca ggacgttgag
     gcggcggcgg gccagggcga ggacggcttg ggtgtggcgt tttccttcgg tgcgttttcg
     gtcgtagtag gtgcgcgagg aggggtcggt gcggatgctg accaaggccg acaggtagca
     ggcgcgcagc aggcgccggt cgtagcgtcg ggggcgtttg aggtttccgc tgatgcggcc
     ggaatctcgt ggtaccggcg ccaggccggc gacgccggcg aggcggtcgg cggaggcgaa
     tgcggccatg tccccgccgg tggcggcgag gaactcagcg cccaggatga cgccgaatcc
     gggcatgctc aggatgattt cggcgtggcg gtggcggcga aatcgctcct cgatcatcgc
     gtcggtgtcg ccgatttcgg tgtcgagggc catcacctcc ttggccaggc gggccaccac
     agtggccgcc agttgttggc cgggcacgat gctgtgttgg gcgttagcgg cctgca
//