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ID   PAM373     preliminary; circular DNA; SYN; 3600 BP.
AC   ATCC43062;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Streptococcus/E.coli plasmid vector pAM373 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pAD1 from Streptococcus faecalis, cAD1 mating response
RC   pAD2 from Streptococcus faecalis, cAD2 mating response
RC   pPD1 from Streptococcus faecalis, cPD1 mating response
RC   pPD2 from Streptococcus faecalis, cPD2 mating response
RC   pPD3 from Streptococcus faecalis, cPD3 mating response
RC   pPD4 from Streptococcus faecalis, cPD4 mating response
RC   pPD5 from Streptococcus faecalis, cPD5 mating response
RC   pPD6 from Streptococcus faecalis, cPD6 mating response
RC   pAMgamma1 from Streptococcus faecalis, cAMgamma1 mating response
RC   pAMgamma2 from Streptococcus faecalis, cAMgamma2 mating response
RC   pAMgamma3 from Streptococcus faecalis, cAMgamma3 mating response
RC   pAMalpha1 from Streptococcus faecalis, cAMalpha1 mating response
RC   pAMbeta1 from Streptococcus faecalis, cAMbeta1 mating response
RC   pAM373 from Streptococcus faecalis RC73, cAM373 mating response
RC   pAM377 from pAM373 & Tn917
RC   pAM378 from pAM373 & Tn918
RC   pAM380 from pAM377 & Tn916
RC   pAMalpha1::Tn917 from pAMalpha1 & Tn917
RC   pAM81 from Streptococcus faecalis, cAM81 mating response
RC   pAM180 from pAM81 & Tn916
RC   pAM360 from pAD1 & Tn918
RC   pAM361 from pAD1 & Tn918
RC   pAM210 from pAD1 & Tn916
RC   pAM170 from pGL101 & Tn916
RA   Clewell D.B., An F.Y., White B.A., Gawron-Burke C.;
RT   "Streptococcus faecalis sex pheromone (cAM373) also produced by
RT   Staphylococcus aureus and identification of a conjugative
RT   transposon (Tn918)";
RL   J. Bacteriol. 162:1212-1220(1985).
RN   [2]
RC   pAM120 from pGL101 & pAM211
RC   pAM120LT from pGL101 & pAD1
RC   pAM170 from pGL101 & pAM210
RC   pAM170LT from pGL101 & pAD1
RC   pAM117 from pACYC184 & pAD1
RC   pAM115 from pACYC184 & pAD1
RC   pAM181 from pAM180 & pACYC177
RC   pAM182 from pAM180 & pACYC177
RC   [pJOE406 from pJOE series]
RC   pAM105 from pJOE406 & pAM120
RC   pAM118 from pVA838 & pAM211
RC   pAM150 from pAM120
RA   Gawron-Burke C., Clewell D.B.;
RT   "Regeneration of insertionally inactivated streptococcal DNA
RT   fragments after excision of Tn916 in Escherichia coli: a strategy
RT   for targeting and cloning of genes from gram-positive bacteria";
RL   J. Bacteriol. 159:214-221(1984).
RN   [3]
RC   pAD1 from Streptococcus faecalis
RC   pAD2 from Streptococcus faecalis
RA   Clewell D.B., Tomich P.K., Gawron-Burke C., Franke A.E., Yagi Y.,
RA   An F.;
RT   "Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and
RT   studies relating to transposition of Tn917";
RL   J. Bacteriol. 152:1220-1230(1982).
RN   [4]
RC   pAM211 from pAD1 & Tn916
RC   pAM210 from pAD1 & Tn916
RA   Franke A.E., Clewell D.B.;
RT   "Evidence for a chromosome-borne resistance transpsoon (Tn916) in
RT   Streptococcus faecalis that is capable of conjugal transfer in the
RT   absence of a conjugative plasmid";
RL   J. Bacteriol. 145:494-502(1981).
RN   [5]
RC   pAM180 from pAM81 & Tn916
RA   Gawron-Burke C., Clewell D.B.;
RT   "A transposon in Streptococcus faecalis with fertility properties";
RL   Nature 300:281-284(1982).
CC   A natural isolate from Streptococcus faecalis. Encodes a mating
CC   response to pheromone cAM373; can harbor Tn918 which confers
CC   tetracycline resistance.
CC   NM (pAM373)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Streptococcus faecalis RC73)(Streptococcus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Streptococcus faecalis RC73, cAM373 mating
FT                   -> pAM373"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   rep_origin      0..0
FT                   /note="ORI Streptococcus pAM373"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;