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ID   PAM401     preliminary; circular DNA; SYN; 10400 BP.
AC   ATCC37429;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Enterococcus/E.coli plasmid vector pAM401 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pGB354 from pIP501
RC   pAM401 from pACYC184 & pGB354
RC   pAM409 from pAM401 & Tn919
RA   Wirth R., An F.Y., Clewell D.B.;
RT   "Highly efficient protoplast transformation system for
RT   Streptococcus faecalis and a new Escherichia coli-S. faecalis
RT   shuttle vector";
RL   J. Bacteriol. 165:831-836(1986).
RN   [2]
RC   pSA3 from pACYC184 & pGB305
RC   pMF1 from pBR322 & S.equisimilis strep gene
RC   pSA31 from pSA3 & pMF1
RA   Dao M.L., Ferretti J.J.;
RT   "Streptococcus-Escherichia coli shuttle vector pSA3 and its use in
RT   the cloning of streptococcal genes";
RL   Appl. Environ. Microbiol. 49:115-119(1985).
RN   [3]
RC   pAM602, pAM603, pAM604, pAM605 from pTV1
RC   pAM610 from pMV163 & pMK20
RC   pAM910 from pAM610
RC   pAM920, pAM925 from pAM910 & pAD1
RA   Smith M.D.;
RT   "Transformation and fusion of Streptococcus faecalis protoplasts";
RL   J. Bacteriol. 162:92-97(1985).
RN   [4]
RC   pGB302, pGB303, pGB304, pGB305, pGB307, pGB308 from pGB301
RC   pGB310, pGB321 from pGB301
RC   pGB351, pGB352, pGB353, pGB354 from pGB301
RC   pGB3022 from pGB301 & S. aureus
RA   Behnke D., Gilmore M.S.;
RT   "Location of antibiotic resistance determinants, copy control and
RT   replication functions on the double-selective streptococcal
RT   cloning vector pGB301";
RL   Mol. Gen. Genet. 184:115-120(1981).
RN   [5]
RC   pIP500 or RIP500 from S.agalactiae R plasmid, tet gene
RC   pIP501 or RIP501 from S.agalactiae R plasmid, cat gene/ery gene
RA   Horodniceanu T., Bouanchaud D.H., Bieth G., Chabbert Y.A.;
RT   "R plasmids in Streptococcus agalactiae (group B)";
RL   Antimicrob. Agents Chemother. 10:795-801(1976).
RN   [6]
RC   pSM19035 from pGB301
RC   pIP2501 from pIP501
RA   Behnke D.;
RT   "[Vector plasmids and the strategy of molecular cloning for
RT   Streptococci]";
RL   Mol. Gen. Mikrobiol. Virusol. 1985:29-30(1985).
RN   [7]
RC   pAMalpha1delta1 from pAMalpha1
RC   pAMalpha1delta1::Tn917 from pAMalpha1::Tn917 & pAMalpha1delta1
RC   pTV1 from pAMalpha1::Tn917 & pBD95
RC   pTV2 from pTV1
RA   Youngman P.J., Perkins J.B., Losick R.;
RT   "Genetic transposition and insertional mutagenesis in Bacillus
RT   subtilis with Streptococcus faecalis transposon Tn917";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:2305-2309(1983).
RN   [8]
RC   pMV120 from S.agalactiae, tet gene
RC   pMV158 from S.agalactiae, tet gene
RC   pMV163 from S.agalactiae, tet gene
RA   Burdett V.;
RT   "Identification of tetracycline-resistant R-plasmids in
RT   Streptococcus agalactiae (group B)";
RL   Antimicrob. Agents Chemother. 18:753-760(1980).
RN   [9]
RC   pVA318 from S.mutans
RC   pVA380-1 from S.ferus
RC   pSM10 from pVA318
RC   pSM10 from pVA380-1
RA   Malke H., Burman L.G., Holm S.E.;
RT   "Molecular cloning in strpetococci: physical mapping of the vehicle
RT   plasmid pSM10 and demonstration of intergroup DNA transfer";
RL   Mol. Gen. Genet. 181:259-267(1981).
RN   [10]
RC   pGB301 from pIP501
RA   Behnke D., Gilmore M.S., Ferretti J.J.;
RT   "Plasmid pGB301, a new multiple resistance streptococcal cloning
RT   vehicle and its use in cloning of the gentamicin/kanamycin resistance
RT   determinant";
RL   Mol. Gen. Genet. 182:414-421(1981).
RN   [11]
RC   pBD95 from pE194 & pBD64
RA   Gryczan T.J., Hahn J., Contente S., Dubnau D.;
RT   "Replication and incompatibility properties of plasmid pE194 in
RT   Bacillus subtilis";
RL   J. Bacteriol. 152:722-735(1982).
CC   Shuttle vector for E.coli/Streptococcus faecalis.
CC   pACYC184 and pGB354 joined at AvaI and SphI sites.
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pAM401)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli DH1)(Enterococcus faecalis)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. S. faecalis/S. agalactiae R plasmid, cat/ery
FT                   -> pIP501 30000bp
FT                   1. pIP501, cat/rep/cop/MLS
FT                   spontaneous deletion mutant
FT                   -> pGB301 9800bp
FT                   1. pGB301 remove BspRI-HindII/HpaI 3600bp, ery gene
FT                   -> pGB354 6400bp [no BspRI, no HindII]
FT                   1. pACYC184 AvaI 4245bp 2921..2921
FT                   T4 DNA polymerase
FT                   2. pGB354 SphI, complete
FT                   T4 DNA polymerase
FT                   -> pAM401 10400bp"
FT   misc_binding    0..0
FT                   /note="SIT unique NcoI-EcoRI-NruI-SalI-SphI-BamHI-
FT                   EcoRV-BstEII-XbaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli miniplasmid p15A"
FT   rep_origin      0..0
FT                   /note="ORI Streptococcus pIP501"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;