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ID   PAMBETA1   preliminary; circular DNA; SYN; 26500 BP.
XX
AC   X17092; S71727;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   Streptococcus/Enterococcus plasmid vector pAM-beta-1 - incomplete.
XX
KW   cloning vector.
XX
OS   Streptococcus faecalis & Enterococcus faecalis
OC   Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
OC   Streptococcaceae.
XX
RN   [1]
RP   1-5100
RC   pAMbeta1 from Streptococcus faecalis
RC   pMTL20E from pAMbeta1 & pMTL20
RC   pMTL21E from pAMbeta1 & pMTL21
RC   pMTL20C from pC194 & pMTL20
RC   pMTL21C from pC194 & pMTL21
RC   pMTL20Cbeta1, pMTL20Cbeta1', pMTL20Cbeta4 from pMTL20C & pAMbeta1
RC   pMTL21Ebeta1, pMTL21Ebeta1' from pMTL21E & pAMbeta1
RC   pMTL502C from pMTL20Cbeta4 & pMTL20C
RC   pMTL502E from pMTL20Cbeta4 & pMTL20E
RA   Swinfield T.J., Oultram J.D., Thompson D.E., Brehm J.K., Minton N.P.;
RT   "Physical characterisation of the replication region of the
RT   Streptococcus faecalis plasmid pAM-beta-1";
RL   Gene 87:79-90(1990).
XX
RN   [2]
RP   1-2129
RC   pMTL531E from pMTL500E & pHV1431
RA   Swinfield T.J., Janniere L., Ehrlich S.D., Minton N.P.;
RT   "Characterization of a region of the Enterococcus faecalis plasmid
RT   pAM beta 1 which enhances the segregational stability of pAM beta
RT   1-derived cloning vectors in Bacillus subtilis.";
RL   Plasmid 26:209-221(1991).
XX
RN   [3]
RC   pCB101 from Clostridium butyricum
RA   Minton N.P., Oultram J.D.;
RT   "Host:vector systems for gene cloning in Clostridium";
RL   Microbiological Science 5:310-315(1988).
XX
RN   [4]
RC   pSM19035 from Streptococcus pyogenes
RC   pCB20, pCB30 from pSM19035, ori
RA   Sorokin A.V., Khazak V.E.;
RT   "Structure of PSM19035 replication region and MLS-resistance gene";
RL   Genetic Tranformation and Expression 0:269-281(1988).
RL   Butler L.O., Harwood C., Moseley B.E., Intercept, Andover UK.
XX
RN   [5]
RC   pR27 from pneumococcus amiA gene & pVA736, ery gene
RC   pR28 from pneumococcus amiA gene & pVA736, ery gene
RC   pR44, pR45 from pR27
RC   pR39 from pR32
RC   pR48 from pR39
RA   Vasseghi H., Claverys J.;
RT   "Amplification of a chimeric plasmid carrying an
RT   erythromycin-resistance determinant introduced into the genome of
RT   Streptococcus pneumoniae";
RL   Gene 21:285-292(1983).
XX
RN   [6]
RC   pAM77 from Streptococcus sanguis
RA   Horinouchi S., Byeon W.H., Weisblum B.;
RT   "A complex attenuator regulates inducible resistance to
RT   macrolides, lincosamides, and streptogramin type B antibiotics in
RT   Streptococcus sanguis";
RL   J. Bacteriol. 154:1252-1262(1983).
XX
RN   [7]
RC   pMTL500E from pAMbeta1 & pMTL20
RC   pLEU100 from pMTL500E & Clostridium pasteurianum leu gene
RA   Oultram J.D., Loughlin M., Swinfield T.J., Brehm J.K., Thompson D.E.,
RA   Minton N.P.;
RT   "Introduction of plasmids into whole cells of Clostridium
RT   acetobutylicum by electroporation";
RL   FEMS Microbiol. Lett. 56:83-88(1988).
XX
RN   [8]
RC   pAMbeta1 from Streptococcus faecalis, ery gene
RA   Clewell D.B., Yagi Y., Dunny G.M., Schultz S.K.;
RT   "Characterization of three plasmid deoxyribonucleic acid molecules
RT   in a strain of Streptococcus faecalis: identification of a plasmid
RT   determining erythromycin resistance";
RL   J. Bacteriol. 117:283-289(1974).
XX
RN   [9]
RC   pAM77 from Streptococcus sanguis
RA   Yagi Y., McClellan T.S., Frez W.A., Clewell D.B.;
RT   "Characterization of a small plasmid determining resistance to
RT   erythromycin , lincomycin, and vernamycin B alpha in a strain of
RT   Streptococcus sanguis isolated from dental plaque";
RL   Antimicrob. Agents Chemother. 13:884-887(1978).
XX
RN   [10]
RC   pR32 from pBR325 & pVA736, ery gene
RA   Mejean V., Claverys J.P., Vasseghi H., Sicard A.M.;
RT   "Rapid cloning of spceific DNA fragments of Streptococcus pneumoniae
RT   by vector integration into the chromosome followed by endonucleolytic
RT   excision";
RL   Gene 15:289-293(1981).
XX
RN   [11]
RC   pKB8 from pSM19035
RA   Sorokin A.V., Vavkov A.S., Bayev V.B., Kozlov Yu.I.;
RT   "Cloning of Bacillus subtilis promoters with the help of
RT   alpha-amylase secretion gene";
RL   Bacillus Molecular Genetics & Biotechnology Application 0:47-54(1988).
RL   Ganesan A.T., Hoch J.A., eds., Academic Press, Orlando FL.
XX
CC   replication region is 5100 bp.
CC   orf H, orf I are 2129 bp.
CC   NM (pAMbeta1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Streptococcus faecalis)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Streptococcus faecalis)
CC   BR ()
CC   OF (pHV1431)(pIP404)(pMTL500E)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Streptococcus faecalis
FT                   -> pAMbeta1"
FT   CDS             <1..476
FT                   /note="GEN Streptococcus ORF A; AA at base 3"
FT   RBS             488..496
FT                   /note="RBS Streptococcus ORF B; putative"
FT   CDS             505..873
FT                   /note="GEN Streptococcus ORF B"
FT   RBS             919..927
FT                   /note="RBS Streptococcus ORF C; putative"
FT   CDS             933..1799
FT                   /note="GEN Streptococcus ORF C"
FT   CDS             2121..2405
FT                   /note="GEN Streptococcus ORF F; TTG start codon"
FT   RBS             2759..2765
FT                   /note="RBS Streptococcus ORF D; putative"
FT   CDS             2772..3071
FT                   /note="GEN Streptococcus ORF D"
FT   RBS             3100..3106
FT                   /note="RBS Streptococcus ORF E; putative"
FT   CDS             3114..4604
FT                   /note="GEN Streptococcus ORF E"
FT   RBS             4942..4948
FT                   /note="RBS Streptococcus ORF G; putative"
FT   CDS             4954..5091
FT                   /note="GEN Streptococcus ORF G; TTG start codon"
FT   CDS             51..665
FT                   /note="GEN Streptococcus ORF H;
FT                   bacterial site-specific recombinase homolog;
FT                   sequence [1]"
FT   CDS             668..2128
FT                   /note="GEN Streptococcus ORF I; partial;
FT                   topoisomerase I homolog; sequence [2]"
XX
SQ   Sequence 7289 BP; 2741 A; 1165 C; 1371 G; 1952 T; 60 other;
     gaattcattt gttcatatct attttgactc tttttattgt attattgata ggaataagtg
     ttattcaaca agtacaactt caatcagtca aaaaagagag cgaattattg gaagaacaaa
     ttgaacgtgt caaagaaacg gatatttctc aatcgaaaat agatactttt ggaagatact
     ttttgactta ctatttttca caggaaaaaa atcaagaaaa ttatcagtca agtttaagaa
     catatgtatc cgaaaaggta gatatatccg attggaaagc gttagggaaa acgcttaaga
     gtgtcaatta ctatggtagt gaacaaacaa aaaaagggta tagcgttgaa taccttctaa
     atgtttcagt agacaatcgt agtaaaatgc aaaaaatcac ttttgaggtt gaaccaacta
     aaaacggctt tcttgtgaca actcaaccaa aacttacaga cttctctttt aactgaaata
     cgctaaaaag agagtgctta aacaatggga aaaattaatt taaatcaaat ttacacagca
     aaagaaatga gtgaaaggat aggtaagaat agaaattacc tatcccaagc ttatcgtaac
     aacaaacatg aaatacttaa aaattttaat tatcgaaaaa ttggtggaac aattattttc
     tctgataatc ccaataatga tttatcgcaa ttgattacag caaaagaagc aagccagttg
     ttaggaaaga atgatgaata ctttgcccac atttataaac ggtttcctca tagactagag
     ggaatcgatc acatttatac aggtaaaaca ttattcttaa cgaaagaatc gctagaagtt
     tttaaaaaaa agatgaacaa aaatgtcaga taaagcatat ttatgatata ctatttatgt
     acacaaatat taatttttaa ggagagaaga aaatgaaaaa aattgccctt ttaacacttt
     ttagtacagc gctattggta tcagccccac ttgttagttt tgcggacgaa gcaacaggta
     gttcaggtgt caatgttcta gctgatgatg tagttgatcc agtagaacca accgatccaa
     ctactccggt tgatccagtt gatccagttg atccagttga tccagttgat ccagttgatc
     cagttgatcc agtcgatcca gttgatccag tcgatccaac agagccaacg gaaccaacag
     agccaacgga accaacagag ccaacggaac caacagagcc aacagagcca acggaaccaa
     cggaaccaac ggaaccaacg gaaccaacag agccaacgga accaacggaa ccaacagagc
     caacggaacc aacagagcca acggaaccaa cggaaccaac agagccaaca gagccaacgg
     aaccaacgga accaacagag ccaacggaac caacagagcc aacagagcca acagagccaa
     cggaaccaac ggagccaacg gaaccaacgg aaccaagcaa gccgacagag ccaacagaac
     caagtaaacc agtagacaca aacccaatcg aaaacccagt taatacagat acaggcgttg
     tgattgttgc agttgaagat agtaaaccaa tcattcaatt agcagacgga acaacaaaaa
     aagttgaagc taaagaaatt ggcgcagacg ttcaaaaaga cggaacagta acggttaaag
     gttcagacgg aaaaatgaaa gttttaccaa aaacaggaga aacagaaaat attgcattgt
     ctgtcctagg ttcattaatg gtattaggat cagcattcat ttttaaaaag agaatctaat
     taagtatttt taaaagcatg gtcgcaagtt tcactagcag ccatgctttt tttgaatctt
     tgtaggacgt tttaaagaga attacccact ttaaaattaa acatgcgctt agaatcgctt
     taggaaacac gatccagtcc aataatcgtc gataaaaact tttgaaaaag gttggtgaaa
     ttacctactt ttggaataat cacaaatcac aagtgattaa tcacaaatca caagtgatta
     atcacttgtt tattaagata ttaaaagcta taatttaaat aaagcgtgaa ttttattaca
     caaaaagagg ggggagaaac ttggaactag catttagaga aagcttaaaa aagatgagag
     gtaccaaatc aaaagaaaaa ttctcccaag aattagaaat gagtagatca aattattcac
     gaatagaatc aggaaaatca gatccaacca taaaaacact agaacaaatt gcaaagttaa
     ctaactcaac gctagtagtg gatttaatcc caaatgagcc aacagaacca gaaccagaaa
     cagaatcaga acaagtaaca ttggatttag aaatggaaga agaaaaaagc aatgacttcg
     tgtgaataat gcacgaaatc gttgcttatt tttttttaaa agcggtatac tagatataac
     gaaacaacga actgaataga aacgaaaaaa gagccatgac acatttataa aatgtttgac
     gacattttat aaatgcatag cccgataaga ttgccaaacc aacgcttatc agttagtcag
     atgaactctt ccctcgtaag aagttattta attaactttg tttgaagacg gtatataacc
     gtactatcat tatataggga aatcagagag ttttcaagta tctaagctac tgaatttaag
     aattgttaag caatcaatcg gaaatcgttt gattgctttt tttgtattca tttatagaag
     gtggagtttg tatgaatcat gatgaatgta aaacttatat aaaaaatagt ttattggaga
     taagaaaatt agcaaatatc tatacactag aaacgtttaa gaaagagtta gaaaagagaa
     atatctactt agaaacaaaa tcagataagt atttttcttc ggagggggaa gattatatat
     ataagttaat agaaaataac aaaataattt attcgattag tggaaaaaaa ttgacttata
     aaggaaaaaa atctttttca aaacatgcaa tattgaaaca gttgaatgaa aaagcaaacc
     aagttaatta aacaacctat tttataggat ttataggaaa ggagaacagc tgaatgaata
     tcccttttgt tgtagaaact gtgcttcatg acggcttgtt aaagtacaaa tttaaaaata
     gtaaaattcg ctcaatcact accaagccag gtaaaagcaa aggggctatt tttgcgtatc
     gctcaaaatc aagcatgatt ggcggtcgtg gtgttgttct gacttccgag gaagcgattc
     aagaaaatca agatacattt acacattgga cacccaacgt ttatcgttat ggaacgtatg
     cagacgaaaa ccgttcatac acgaaaggac attctgaaaa caatttaaga caaatcaata
     ccttctttat tgattttgat attcacacgg caaaagaaac tatttcagca agcgatattt
     taacaaccgc tattgattta ggttttatgc ctactatgat tatcaaatct gataaaggtt
     atcaagcata ttttgtttta gaaacgccag tctatgtgac ttcaaaatca gaatttaaat
     ctgtcaaagc agccaaaata atttcgcaaa atatccgaga atattttgga aagtctttgc
     cagttgatct aacgtgtaat cattttggta ttgctcgcat accaagaacg gacaatgtag
     aattttttga tcctaattac cgttattctt tcaaagaatg gcaagattgg tctttcaaac
     aaacagataa taagggcttt actcgttcaa gtctaacggt tttaagcggt acagaaggca
     aaaaacaagt agatgaaccc tggtttaatc tcttattgca cgaaacgaaa ttttcaggag
     aaaagggttt aatagggcgt aataacgtca tgtttaccct ctctttagcc tactttagtt
     caggctattc aatcgaaacg tgcgaatata atatgtttga gtttaataat cgattagatc
     aacccttaga agaaaaagaa gtaatcaaaa ttgttagaag tgcctattca gaaaactatc
     aaggggctaa tagggaatac attaccattc tttgcaaagc ttgggtatca agtgatttaa
     ccagtaaaga tttatttgtc cgtcaagggt ggtttaaatt caagaaaaaa agaagcgaac
     gtcaacgtgt tcatttgtca gaatggaaag aagatttaat ggcttatatt agcgaaaaaa
     gcgatgtata caagccttat ttagtgacga ccaaaaaaga gattagagaa gtgctaggca
     ttcctgaacg gacattagat aaattgctga aggtactgaa ggcgaatcag gaaattttct
     ttaagattaa accaggaaga aatggtggca ttcaacttgc tagtgttaaa tcattgttgc
     tatcgatcat taaagtaaaa aaagaagaaa aagaaagcta tataaaggcg ctgacaaatt
     cttttgactt agagcataca ttcattcaag agactttaaa caagctagca gaacgcccta
     aaacggacac acaactcgat ttgtttagct atgatacagg ctgaaaataa aacccgcact
     atgccattac atttatatct atgatacgtg tttgtttttt ctttgctgtt tagcgaatga
     ttagcagaaa tatacagagt aagattttaa ttaattatta gggggagaag gagagagtag
     cccgaaaact tttagttggc ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg
     aggggcagtg agagcgaagc gaacacttga ttttttaatt ttctatcttt tataggtcat
     tagagtatac ttatttgtcc tataaactat ttagcagcat aatagattta ttgaataggt
     catttaagtt gagcatatta gaggaggaaa atcttggaga aatatttgaa gaacccgatt
     acatggattg gattagttct tgtggttacg tggtttttaa ctaaaagtag tgaatttttg
     atttttggtg tgtgtgtctt gttgttagta tttgctagtc aaagtgatta aatagaattc
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     gaattcatat ccaatttatt tttttcttaa caagggaggt gttttttaac atgactaaag
     tagggtatgc acgtgtcagt agcaaagaac agaacttaga tagacaactg aaagcgttag
     agggcgtttc taaggtcttt tcagacaaag caagcggtca atcggtcgaa cgcccacaat
     tacaagctat gcttaactat attcgtgaag gggatatagt tgttgttact gaattagatc
     gattaggacg aaataataaa gaattaacag aattgatgaa tcaaattcaa attaaggggg
     caaccctgga agtcttaaat ttaccctcaa tgaatggtat tgaagatgaa aatttaagac
     ggctgattaa taatttagtg attgaattgt ataagtacca accggaatct gaacgcaaac
     gaattaaaga acgccaagcc caaggaattg aaattgctaa gaaaaaagga aaattcaaag
     ggcgacaact gaaattcaaa gaaaatgatc cacgtttaca acacgctttc gatttgtttt
     tgaacggttt atccgataaa gaagttgaag aacaaactgg aattaatcgc cgaacgttta
     gaaggtatcg atcaagatac aacgtgacag tcgatcaaag aaaaaacaat gaaaagaggg
     atagttaatg agtacggtta ttttagctga aaaaccaagc caggcattag cctatgcaag
     tgctttaaaa caaagcacca aaaaagactg ttattttgag atcaaagacc caatctttgc
     agatgaaacg tttatcacgt ttggttttgg gcatttagtc gagttagcag aaccaggtca
     ttatgacgaa aagtggcaaa attggaaact tgaatcattg ccgatttttc ctgatcgata
     cgattttgaa gtggcaacag ataaaaaaaa gcagtttaaa attgttgctg aacttttaaa
     acaagcaaat acaatcattg tcgcaacaga tagcgacaga gaaggcgaaa acattgcctg
     gtcgatcatt cataaagcaa atgccttttc taaagataaa acgtataaaa gactatggat
     caatagttta gaaaaagatg tgatccgtag cggttttcaa aatttgcaac caggaatgaa
     ttactatccc ttttatcaag aagcgcaaac acgccaaatt gccgattggt tgatcggcat
     gaatgcaagc cctttgtata cgttaaattt acagcagaag ggcgtacaag gtacattttc
     actaggacgt gttcaaacgc ccaccttata tcttattttt cagcgccagg aagccataga
     aaactttaga aaagaacctt ttttcgaggt ggaagctagt ataaaagtaa accaagggtc
     atttaagggc gttataagcc ccacacagcg ctttaaaacc caagaggagc ttttagcttt
     tgtttcttct gaacaagcta aaataggcaa tcaagagggg ataattgctg atgttcaaac
     caaagagaag aaaacgaata gtccgagttt gttttcttta agtagtttgc aatcaaaagt
     taatcagctt tataaagcga cagcgagcca aactttaaaa gctatgcaag gactgtatga
     agcaaaatta ttgagttatc caagaacaga tacaccattt attacagaga acgaatttgc
     ttatttaaaa gcgaattttg gcaaatatag cggtttttta ggacttgatc ttgaaatggt
     tcaaacagag cctagaaagc gttatgtgga cggtagtaag gtacaggaac accacgccat
     tatcccaaca aaacaagtac ctaccgaatc tgcattagcg aaaatggacg atttacaacg
     aaaaatatat gctttagtcg ttaaaacgac cgttgccatg tttctacctg attatttgta
     tgaagaaact aagatacaaa ccaaagtagc cgacttactt tttcaatcaa taggcaagac
     accaaagcaa gaaggttgga aaattctttt caaacaacaa accaaagaag aagaagagga
     cgttcaaacg ttacccttgg ttatcattgg agaacatgcc gaggttgacg ttaagagtgc
     cgaaaaagaa acacaaccac cgaaagctt
//