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ID   PAP2034    preliminary; circular DNA; SYN; 8500 BP.
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AC   ATCC37428;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Higher plants/E.coli plasmid vector pAP2034 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAK2004 from pAK1003
RC   pNS1171 from pAK2004 & pLGV1103, Tn1831
RC   pAS2021 from pNS1171 & pOP44392
RC   pAP2034 from pAS2021 & plant g7 polyA
RC   pAS2022 from pAS2021
RC   pAS2023 from pAS2022 & linker
RC   pCAP212, pCAP009 from pAP2034 & cat gene
RA   Velten J., Schell J.;
RT   "Selection-expression plasmid vectors for use in genetic
RT   transformation of higher plants";
RL   Nucleic Acids Res. 13:6981-6998(1985).
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CC   Contains both a selectable marker for plant transformation (kanR) and
CC   a second, directly adjacent, divergent promoter for the transcription
CC   of inserted DNA fragments.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAP2034)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(higher plants)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAK1003 SalI
FT                   2. pACYC177 HaeII-HaeII 1430bp 1596..3026, Tn903 kan
FT                   -> pAK2004
FT                   1. AcMNPV, NOS promoter
FT                   2. Tn1831, strep/spec genes/#?
FT                   -> pLGV1103
FT                   1. pAK2004 SalI-SalI 1430bp, Tn903 kan gene/#V00359
FT                   fill in:fill in
FT                   2. pLGV1103 BamHI-HindIII 2400bp, Tn1831 strep/spec
FT                   fill in:fill in
FT                   -> pNS1171 3600bp
FT                   1. pUC8 SmaI 2665bp 238..238, MCS
FT                   2. pTiAch5 AluI-AluI 676bp, 1'-2' intergenic region
FT                   -> pAR1200 [unique ClaI at init codon of 2' ORF]
FT                   1. pAR1200 ClaI-HindIII, 1' to pUC8 HindIII 261
FT                   2. pBR322 remove ClaI-HindIII 5bp 25..30, 4356bp
FT                   -> pOP1
FT                   1. pOP1 HindIII
FT                   BAL31 nuclease
FT                   BamHI
FT                   Klenow
FT                   -> plasmid [BamHI]
FT                   1. plasmid EcoRI-BamHI
FT                   2. pUC8 remove EcoRI-BamHI 10bp 231..241, MCS/2655bp
FT                   -> pOP443 [BamHI 3bp 5' to start codon]
FT                   1. pOP443
FT                   -> pOP4439
FT                   1. pOP4439
FT                   -> pOP44392
FT                   1. pNS1171 EcoRI-BclI 3600bp
FT                   2. pOP44392 EcoRI-BamHI, 1'-2' intergenic region
FT                   -> pAS2021
FT                   1. pAS2021 remove EcoRI-SalI, MCS
FT                   2. plant EcoRI-SalI, g7 polyA
FT                   -> pAP2034 8500bp"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-SalI-ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SalI-ClaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO A. tumefaciens T-DNA gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli spectinomycin resistance gene
FT                   (spc)"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str)"
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SQ   Sequence 252 BP; 78 A; 49 C; 34 G; 91 T; 0 other;
     cttccacacg tggatcgatc cgtcgacgga tcccccgatg agctaagcta gctatatcat
     caatttatgt attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct
     gatataatca gttattgaaa tatttctgaa tttaaacttt gcatcaataa attatgtttt
     tgcttggact ataatacctg acttatttta tcaataaata tttaaactat atttctttca
     agatgggaat tc
//