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ID   PAR3       preliminary; circular DNA; SYN; 3320 BP.
AC   ATCC87026;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid pAR3 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pAR3 from p15A & E. coli cat gene & E. coli, ara
RA   Cagnon C., Valverde V., Masson J.M.;
RT   "A new family of sugar-inducible expression vectors for
RT   Escherichia coli";
RL   Protein Engineering 4:843-847(1991).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BglII--3.4; EcoRV--2.5, 0.45 (doublet); PstI--3.4. (ATCC staff)
CC   This vector is compatible with ColE1-derived plasmids and allows
CC   efficient expression of recombinant genes upon induction with
CC   arabinose.
CC   These features make it convenient to be used in combination with
CC   standard gene expression vectors for the independently controlled
CC   production of two recombinant polypeptides in E. coli.
CC   (personal communication)
CC   Growth: LB plus chloramphenicol (25 ug/ml) 37C
CC   Deposited by: Gutierrez J.N.
CC   NM (pAR3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(E.coli DH1)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. p15A
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   3. E. coli, paraB promoter/araC gene
FT                   -> pAR3 3320bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli p15A"
FT   CDS             0..0
FT                   /note="ANT E. coli cat gene"
FT   misc_binding    0..0
FT                   /note="MCS NcoI-PstI-NarI-EcoRV-BglII-KpnI-HindIII"
FT   promoter        0..0
FT                   /note="PRO E. coli paraB"
FT   CDS             0..0
FT                   /note="GEN E. coli araC"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;