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ID   PARC13     preliminary; circular DNA; SYN; 1 BP.
AC   IG5137; M35117;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pARC13 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pYEJ001 from pBR327 & lac operators & RNA polymerase promoter
RC   pARC13-1 from pARC13 & pARC1
RC   phage from M13mp18 & pARC13-1
RC   pSEV1 from phage & pIJ702
RC   pSEV2 from phage & pAFS1
RC   pSEV3 from pSEV1 & linker
RC   pSEV4 from pSEV2 & linker
RC   pHEM340 from M13mp18 & pBR322
RC   [pTN13 from pBR327 & T.flavus mdh gene]
RC   pHEM341 from pHEM340 & pTN13
RC   pMDF1 from pHEM341 & pSEV2
RC   pCES1 from pSEV2 & pBC501
RC   [pAFS1 from pKS11 & strep gene]
RA   Horinouchi S., Nishiyama M., Nakamura A., Beppu T.;
RT   "Construction and characterization of multicopy expression-vectors in
RT   Streptomyces spp";
RL   Mol. Gen. Genet. 210:468-475(1987).
RN   [2]
RC   pIJ41-SR51 from pIJ41 & S.lavendulae 1080, strep gene
RC   pTA4001 from S.lavendulae 1080
RC   pKS2, pKS4, pKS5, pKS7, pKS9, pKS10, pKS11, pKS12, pKS13 from pTA4001
RC   pKST2 from pKS7
RA   Kobayashi T., Shimotsu H., Horinouchi S., Uozumi T., Beppu T.;
RT   "Isolation and characterization of a pock-forming plasmid pTA4001
RT   from Streptomyces lavendulae";
RL   J. Antibiot. 37:368-375(1984).
RN   [3]
RC   pIJ41-B1, pIJ41-B2, pIJ41-B3 from pIJ41 & S.coelicolor chromosome
RC   pIJ41-B4, pIJ41-B5 from pIJ41 & S.coelicolor chromosome
RC   pIJ41-B6 from pIJ41-B2
RC   pARC1 from pIJ41-B6 & pIJ41-B5
RC   pARC3 from pARC1 & pSR203, ermC promoter
RC   pARC7 from pARC1 & pDR540, tac promoter
RC   from pARC1 & pBGPC3 or pSGPC3
RC   pARC4 from pARC1 & afsA gene
RC   pARC9, pARC10, pARC11, pARC12, pARC13, pARC14 from pARC1 & S.griseus
RC   pARC15, pARC16, pARC17, pARC18 from pARC1 & S.lividans
RA   Horinouchi S., Beppu T.;
RT   "Construction and application of a promoter-probe plasmid that
RT   allows chromogenic identification in Streptomyces lividans";
RL   J. Bacteriol. 162:406-412(1985).
RN   [4]
RC   pTA101 from pBR322 & T.flavus mdh gene
RC   pTM'1 from pBR322 & T.flavus mdh gene
RC   pTM1, pTM4, pTM5 from pTA101
RC   pTM7 from pTM1 & pYEJ001 & linker
RC   pTM'7 from pTM'1 & pTM7
RC   pTM'3, pTM'4, pTM'5, pTM'6 from pTM'7
RA   Nishiyama M., Matsubara N., Yamamoto K., Iijima S., Uozumi T.,
RA   Beppu T.;
RT   "Nucleotide sequence of the malate dehydrogenase gene of Thermus
RT   flavus and its mutation directing an increase in enzyme activity";
RL   J. Biol. Chem. 261:14178-14183(1986).
RN   [5]
RC   pBC5 from pBD64 & B.subtilis cellulase gene
RC   pBC501 from pUB110 & pBC5 & linker
RA   Nakamura A., Uozumi T., Beppu T.;
RT   "Nucleotide sequence of a cellulase gene of BAcillus subtilis";
RL   Eur. J. Biochem. 164:317-320(1987).
RN   [6]
RC   pSGPC3 or pBGPC3 from yeast GAL7 gene
RA   Yamashita T., Beppu T.;
RT   ;
RL   Unpublished (1985).
RN   [7]
RC   pSR203 from pHW1, ermC promoter
RA   Tsuchiya M.;
RT   ;
RL   Unpublished (1985).
RN   [8]
RC   pSV1 from Streptomyces
RA   Aguilar A., Hopwood D.A.;
RT   "Determination of methylenomycin A synthesis by the pSV1 plasmid
RT   from Streptomyces violaceus-ruber SANK 95570";
RL   J. Gen. Microbiol. 128:1893-1901(1982).
CC   promoter. gene. in GenBank.
CC   NM (pARC13)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pARC1 from pBR322)(pTN13 from pBR327)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Streptomyces
FT                   -> SLP1
FT                   1. SLP1
FT                   -> SLP1.2
FT                   1. SLP1.2
FT                   2. S. fradiae aph gene
FT                   -> pIJ2
FT                   1. SLP1.2
FT                   2. S. azureus tsr gene
FT                   -> pIJ6
FT                   1. pIJ2
FT                   -> pIJ37
FT                   1. pIJ6
FT                   2. pIJ37
FT                   -> pIJ41
FT                   1. pIJ41 remove small BamHI-XhoI
FT                   2. S.coelicolor chromosome BamHI-XhoI 6700bp
FT                   -> pIJ41-B2 [no pigment]
FT                   1. pIJ41 BamHI
FT                   2. S.coelicolor chromosome BamHI-BamHI 6700bp
FT                   -> pIJ41-B5 [no pigment]
FT                   1. pIJ41-B2 remove BamHI-BamHI 2300bp
FT                   BAL31 nuclease
FT                   -> pIJ41-B6
FT                   1. pIJ41-B6 EcoRI-XhoI
FT                   2. pIJ41-B5 EcoRI-XhoI
FT                   -> pARC1 19000bp [unique BamHI]
FT                   1. pARC1
FT                   2. S.griseus 1600bp, promoter
FT                   -> pARC13
FT                   1. pARC13 PstI-XhoI, transcriptional signal
FT                   Sau3A-Sau3A 100bp, transcriptional signal
FT                   2. pARC1 BamHI
FT                   -> pARC13-1"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;