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ID   PARC5      preliminary; circular DNA; SYN; 4400 BP.
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AC   ATCC37352;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pARC5 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pARC5 from Charon 34 & pBR322
RC   pARC6 from pSV1 or pBR322SV & linker
RC   pARC7 from pARC5 & pARC6
RA   Alexander D.C., McKnight T.D., Williams B.G.;
RT   "A simplified and efficient vector-primer cDNA cloning system";
RL   Gene 31:79-89(1984).
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CC   This is a component of a vector-primer cDNA cloning system to be used
CC   with pARC7 (ATCC 37353).
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pARC5)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> Charon 10 [lambda lac5 deltaH3 bio256 KH54 nin5]
FT                   1. lambda
FT                   -> Charon 13A [lambda b1007 KH54 nin5 QSR imm80]
FT                   1. lambda
FT                   -> Charon 14 [lambda lac5 b1007 KH54 nin5 QSR imm80]
FT                   1. Charon 21
FT                   -> Charon 21A [lambda b1007 imm80 KH53 KH52 nin5]
FT                   1. Charon 14
FT                   cross with
FT                   2. Charon 21A
FT                   -> lambda BP1 [lambda lac5 b1007 imm80 KH53 KH52 nin5]
FT                   1. lambda BP1
FT                   mutant
FT                   -> lambda BVC4 [no BamHI 5' to lac5]
FT                   1. Charon 21A
FT                   cross with
FT                   2. lambda BVC4
FT                   -> Charon 27 [lambda b1007 imm80 KH53 KH52 nin5; Bam-]
FT                   1. Charon 13A
FT                   cross with
FT                   2. Charon 10
FT                   -> lambda XDR3.24 [lambda b1007 KH54 nin5]
FT                   1. lambda XDR3.24
FT                   cross with
FT                   2. lambda BVC4
FT                   -> Charon 28-2221 [lambda b1007 KH54 nin5; Bam-]
FT                   1. Charon 28-2221
FT                   mutation
FT                   -> Charon 28-2222 [lambda b1007 KH54 nin5 ?; Bam-]
FT                   1. Charon 28-2221
FT                   mutation
FT                   -> Charon 28-2270 [lambda ? b1007 KH54 nin5; Bam-]
FT                   1. Charon 28-2222
FT                   2. Charon 28-2270
FT                   -> Charon 30 [lambda b1007 b1007 KH54 nin5; Bam-]
FT                   1. Charon 30
FT                   -> Charon 30A [lambda b1007 b1007 KH54 nin5; Bam-]
FT                   1. piAN7 SalI-BamHI
FT                   DNA polymerase I:
FT                   2. lambda Sau3A-AluI 18783..19159
FT                   -> phage [SalI at 19159 instead of AluI]
FT                   1. phage, plasmid and SupF
FT                   recombination
FT                   2. Charon 30A, arms
FT                   -> lambda FAC101 [SalI at 19159 instead of AluI]
FT                   1. Charon 30
FT                   cross with
FT                   2. lambda BVC4
FT                   -> Charon 31 [lambda lac5 b1007 b1007 KH54 nin5]
FT                   1. Charon 31 remove EcoRI-EcoRI
FT                   2. mouse liver DNA EcoRI-EcoRI 12000bp [no BamHI,SalI]
FT                   -> Charon 32 [unique BamHI]
FT                   1. Charon 32 BamHI-SalI
FT                   Klenow:Klenow
FT                   -> Charon 33 [unique SalI, no BamHI, in right arm]
FT                   1. pUC13 BamHI 2680bp 261..261
FT                   Klenow
FT                   -> pUC13Bam- 2680bp
FT                   1. lambda 540
FT                   2. E. coli HindIII-HindIII 10000bp, lpp gene
FT                   -> lambda lppEc-1
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. lambda lppEc-1 HaeIII-HaeIII 2800bp, lpp gene
FT                   EcoRI linker 8bp ngaattcn:EcoRI linker 8bp ngaattcn
FT                   -> pKEN111
FT                   1. pKEN111
FT                   2. pBR322
FT                   -> pKEN033
FT                   1. pKEN033
FT                   -> pKEN037
FT                   1. pMB9 EcoRI
FT                   DNA polymerase I
FT                   2. lambda h80 lacUV5 cI857 HaeIII-HaeIII 203bp, lacI/Z
FT                   -> pOP203-1
FT                   1. pMB9 EcoRI
FT                   DNA polymerase I
FT                   2. lambda h80 lacUV5 cI857 HaeIII-HaeIII 203bp, lacI/Z
FT                   -> pOP203-2
FT                   1. pOP203-2 HindIII-HaeIII 530bp, lacUV5 promoter
FT                   2. pMB9 EcoRI
FT                   DNA polymerase I
FT                   HindIII
FT                   -> pOP203-3 [unique EcoRI near UV5]
FT                   1. pKEN037
FT                   2. pOP203-3
FT                   -> pIN-II-A1
FT                   1. pUC13Bam- HindIII 2680bp 234..234
FT                   2. pIN-II-A1 HindIII, complete
FT                   -> plasmid2
FT                   1. plasmid2 EcoRI-EcoRI, MCS
FT                   1. Charon 33 EcoRI
FT                   -> Charon 34 [EcoRI-...-HindIII-BamHI-EcoRI]
FT                   1. Charon 34 BamHI-SstI, MCS
FT                   :dC tailing
FT                   BamHI-SstI-dCG-SstI-BamHI, MCS inverted repeat
FT                   2. pBR322 BamHI 4361bp 376..376, tet
FT                   -> pARC5 4400bp"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-HindIII-PstI-SalI-XbaI-SmaI-SstI-
FT                   SmaI-XbaI-SalI-PstI-HindIII-BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI-HindIII-PstI-SalI-
FT                   XbaI-SmaI-SstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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