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ID   PARC7      preliminary; circular DNA; SYN; 6300 BP.
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AC   ATCC37353;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pARC7 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pARC5 from Charon 34 & pBR322
RC   pARC6 from pSV1 or pBR322SV & linker
RC   pARC7 from pARC5 & pARC6
RA   Alexander D.C., McKnight T.D., Williams B.G.;
RT   "A simplified and efficient vector-primer cDNA cloning system";
RL   Gene 31:79-89(1984).
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CC   This is a component of a vector-primer cDNA cloning system to be used
CC   with pARC5 (ATCC 37352).
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pARC7)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove PvuII-HindIII 2047bp 30..2067,
FT                   \ tet/2314bp
FT                   2. SV40 PvuII-HindIII 774bp 273..1047
FT                   \ late polyA/786bp/0.71 to 0.86
FT                   -> pBR322-SV40 3088bp
FT                   1. pBR322-SV40 3088bp
FT                   2. pBR322-SV40 3088bp
FT                   -> pBR322-SV40 dimer 6176bp
FT                   1. pBR322-SV40 dimer KpnI 6176bp, SV40 299/SV40 299
FT                   T4 DNA polymerase
FT                   BamHI linker 6bp ggatcc
FT                   -> pARC6 6176bp
FT                   1. pARC5 HindIII-HindIII 100bp, pUC13 MCS/pBR322 30
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pARC6 KpnI 6176bp, SV40 299
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pARC7 6300bp"
FT   misc_binding    0..0
FT                   /note="MCS PstI-SalI-XbaI-SmaI-SstI-SmaI-XbaI-SalI-
FT                   PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI-SalI-XbaI-SmaI-SstI-BamHI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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