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ID   PARO180    preliminary; circular DNA; SYN; 5460 BP.
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AC   ATCC77123;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Enterobacter/E.coli plasmid vector pARO180 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pARO160 from pUC18 & pSUP2021
RC   pARO161 from pARO160 & pBR325, Tn5 kan gene
RC   pARO163 from pUC18 & pARO161
RC   pARO173 from pUC19 & pARO161
RC   pARO190 from pUC19 & pARO173
RC   pARO191 from pARO173
RC   pARO181 from pARO191 & pARO163
RC   pARO180 from pARO190 & pUC18
RA   Parke D.;
RT   "Construction of mobilizable vectors derived from plasmids RP4,
RT   pUC18 and pUC19";
RL   Gene 93:135-137(1990).
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CC   Mobilizable expression vector encoding the lacZalpha (lacZ') peptide
CC   permitting visual detection of recombinants. Contains priming sites
CC   useful for sequencing.
CC   Can be used as an expression vector in bacteria of the family
CC   Enterobacteriaceae, or as a mobilizable suicide vector for
CC   site-specific insertional mutagenesis in diverse other bacterial
CC   strains.
CC   Replicates to high copy number in E.coli and Enterobacter aerogenes.
CC   Contains the basis of mobilization (bom) and origin of transfer (oriT)
CC   sequences from RP4.  This region can be transferred to another version
CC   of pUC vectors by using a MCS restriction site and the unique AlwNI
CC   site.
CC   The order of the major features in this plasmid is:lacZ'/MCS -
CC   pMB1 ori - AlwNI site - bla - oriT/bom. pARO180 (ATCC 77123) and
CC   pARO190 (ATCC 77124)
CC   differ in the orientation of the MCS in lacZ'.
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--5.5; PstI--5.5; BamHI--5.5; EcoRI--5.5; HindIII/EcoRI--5.5.
CC   (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pARO180)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DH5alpha)(Enterobacter aerogenes)(E.coli)
CC   HO (E.coli S17-1 [ATCC 47055])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 AlwNI-SspI 1281bp 1223..2504, amp
FT                   2. pARO190 SspI-AlwNI 4765bp, pUC19 2504..2686..1223
FT                   -> pARO180 6046bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-AccI-HincII-SalI-XbaI-
FT                   BamHI-AvaI-SmaI-XmaI-KpnI-BanII-SacI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-SphI-PstI-HincII-SalI-
FT                   XbaI-BamHI-AvaI-SmaI-XmaI-KpnI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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