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ID   PAS1       preliminary; circular DNA; SYN; 7100 BP.
AC   ATCC87007;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli phagemid pAS1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pACT, pACT2 from LEU2 gene
RA   Durfee T., Becherer K., Chen P.L., Yeh S.H., Yang Y., Kilburn A.E.,
RA   Lee W.H., Elledge S.J.;
RT   "The retinoblastoma protein associates with the protein
RT   phosphatase type 1 catalytic subunit";
RL   Genes Dev. 7:555-569(1993).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--7.2; SalI--7.2; SmaI--7.2. (ATCC staff)
CC   Shuttle expression vector used to create fusion proteins
CC   consisting of the nuclear localization sequence from SV40 T antigen,
CC   the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin)
CC   epitope tag in frame with the activation domain.
CC   The order of the major features in this plasmid is:
CC   ADC1 (ADH) promoter -> - GAL4 DNA binding domain - HA -
CC   EcoRI - NdeI/MCS/SalI - ADC1 terminator - pMB1 ori - ampR -
CC   2 micron ori - TRP1 -> - f1 ori. [1]
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: Elledge S.J.
CC   NM (pAS1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   HO (E.coli)(Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)(expression)(shuttle - episomal)(DNA library)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp
FT                   2. SV40, T antigen
FT                   3. HA epitope
FT                   4. yeast, GAL4
FT                   5. yeast, TRP1
FT                   -> pAS1 7100bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   rep_origin      0..0
FT                   /note="ORI E. coli phage f1"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   misc_binding    0..0
FT                   /note="MCS NdeI-NcoI-SfiI-SmaI-BamHI-SalI"
FT   promoter        0..0
FT                   /note="PRO yeast ADH/ADC1"
FT   misc_feature    0..0
FT                   /note="GAL4 activation domain"
FT   misc_feature    0..0
FT                   /note="HA (hemagglutinin) epitope tag"
FT   terminator      0..0
FT                   /note="TER yeast ADC1"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;