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ID   PAS2023    preliminary; circular DNA; SYN; 8300 BP.
AC   ATCC37427;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Higher plants/E.coli plasmid vector pAS2023 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pAK1003 from pTiAch5
RC   pAK2004 from pAK1003
RC   pNS1171 from pAK2004 & pLGV1103, Tn1831
RC   pAS2021 from pNS1171 & pOP44392
RC   pAP2034 from pAS2021 & g7 polyA
RC   pAS2022 from pAS2021
RC   pAS2023 from pAS2022 & linker
RC   pCAP212, pCAP009 from pAP2034 & cat gene
RA   Velten J., Schell J.;
RT   "Selection-expression plasmid vectors for use in genetic
RT   transformation of higher plants";
RL   Nucleic Acids Res. 13:6981-6998(1985).
CC   Contains both a selectable marker for plant transformation (kanR) and
CC   a second, directly adjacent, divergent promoter for the transcription
CC   of inserted DNA fragments.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAS2023)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(higher plants)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pAS2022 remove EcoRI-SalI, MCS/pBR322 8300bp
FT                   2. pUC8 EcoRI-SmaI-BamHI-SalI 16bp 231..247, MCS
FT                   -> pAS2023 8300bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-SalI-ClaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO A. tumefaciens T-DNA gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli spectinomycin resistance gene
FT                   (spc)"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str)"
SQ   Sequence 43 BP; 8 A; 15 C; 11 G; 9 T; 0 other;
     cttccacacg tggatcgatc cgtcgacgga tccccgggaa ttc