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ID   PAT112     preliminary; circular DNA; SYN; 4900 BP.
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AC   IG5064; S66277;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pAT112 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAT112 from pACYC184 & RK2 & pJH1 & Tn1545
RC   pAT113 from pAT112 & pUC19
RC   pAT113 from pAT112 & pUC18
RA   Trieu-Cuot P., Carlier C., Poyart-Salmeron C., Courvalin P.;
RT   "An integrative vector exploiting the transposition properties of
RT   Tn1545 for insertional mutagenesis and cloning of genes from
RT   gram-positive bacteria";
RL   Gene 106:21-27(1991).
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RN   [2]
RC   pJH1 or pIP800 from Streptococcus faecalis, kan gene/cat gene
RC   pAT21 from pBR322 & pJH1, Streptococcus kan gene
RC   pAT21.1 from pAT21
RC   pPJ1 from pJH1, Streptococcus kan gene
RA   Trieu-Cuot P., Courvalin P.;
RT   "Nucleotide sequence of the Streptococcus faecalis plasmid gene
RT   encoding the 3'5'-aminoglycoiside phosphotransferase type III";
RL   Gene 23:331-341(1983).
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RN   [3]
RC   Tn1545 from S. pneumoniae, ery gene
RC   pAT110 from pUC18 & Tn1545
RA   Trieu-Cuot P., Poyart-Salmeron C., Carlier C., Courvalin P.;
RT   "Nucleotide sequence of the erythromycin resistance gene of the
RT   conjugative transposon Tn1545";
RL   Nucleic Acids Res. 18:3660-3660(1990).
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RN   [4]
RC   pIP800 or pJH1 from Streptococcus faecalis, kan gene/cat gene
RC   pIP801 from Streptococcus faecalis, ery gene
RC   pIP802 from Streptococcus faecalis
RC   pIP803 from Streptococcus faecalis, tet gene
RC   pIP800-1 from Streptococcus faecalis, cat gene
RA   Courvalin P., Carlier C., Collatz E.;
RT   "Plasmid-mediated resistance to aminocyclitol antibiotics in group
RT   D streptococci";
RL   J. Bacteriol. 143:541-551(1980).
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RN   [5]
RC   pIP1527 from E. coli
RA   Brisson-Noel A., Arthur M., Courvalin P.;
RT   "Evidence for natural gene transfer from gram-positive cocci to
RT   Escherichia coli";
RL   J. Bacteriol. 170:1739-1745(1988).
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CC   attTn1545
CC   NM (pAT112)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pACYC184)(RK2)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. S. pneumoniae, ery gene
FT                   -> Tn1545
FT                   1. Streptococcus faecalis, kan gene/cat gene
FT                   -> pJH1 72600bp
FT                   1. pACYC184 EcoRI-XbaI 1424bp 2..1426, oriR
FT                   2. RK2 PstI-SmaI 700bp, oriT
FT                   3. pJH1 ClaI-HindIII 1300bp, kan gene
FT                   4. Tn1545 BamHI-KpnI 1300bp, ery gene
FT                   5. Tn1545 KpnI-EcoRI 320bp, attTN1545 termini
FT                   -> pAT112 4900bp [unique AvaI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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