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ID   PATH101    preliminary; circular DNA; SYN; 400 BP.
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AC   S49970;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pATH10.1 - incomplete, trpE promoter.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-400
RC   pATH2.1 from pATH2 & oligo
RC   pATH10.1 from pATH10 & oligo
RA   Mercy M.R., Troncoso J.C., Monteiro M.J.;
RT   "A new series of trpE vectors that enable high expression of
RT   nonfusion proteins in bacteria";
RL   Protein Expr. Purif. 3:57-64(1992).
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RN   [2]
RC   plasmid from pBR322 & S.aureus nuc gene
RC   pFOG301 from plasmid
RA   Shortle D.;
RT   "A genetic system for analysis of staphylococcal nuclease";
RL   Gene 22:181-189(1983).
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CC   promoter. in GenBank.
CC   NCBI gi: 259139
CC   NM (pATH10.1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pATH10)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pATH10 remove PvuII-EcoRI 1394bp 4..1398,
FT                   \ trpE 2377bp
FT                   2. pATH10 PvuII-EcoRI 423bp 1398..PCR..4
FT                   \ tcaccgaaacgcgcgag...
FT                   \ ...cgcgaattcgcgacattgttattctctaat
FT                   PCR
FT                   -> pATH10.1 2800bp"
FT   CDS             398..400
FT                   /note="GEN E. coli trpE gene;
FT                   partial, only start codon shown"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 400 BP; 112 A; 90 C; 99 G; 99 T; 0 other;
     gccagggtgc cgacgcgcat ctcgactgca cggtgcacca atgcttctgg cgtcaggcag
     ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc
     aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg atctggcaaa
     atatctgaaa tgagctgttg acaattaatc atcgaactag ttaactagta cgcaagttca
     cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg aaaggttggt ggcgcacttc
     ctgaaacggg cagtgtattc accatgcgta aagcaatcag atacccagcc cgacgctaat
     gagcgggctt ttttttgaac aaaattagag aataacaatg
//