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ID   PAU9       preliminary; circular DNA; SYN; 4400 BP.
AC   ATCC37799;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Schizosaccharomyces/E.coli plasmid vector pAU9 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pAU5 from pBR322 & pFL20
RC   pAU9 from pBR322 & pFL20
RC   pAU21 from pBR322 & pFL20
RC   pAL7 from pBR322 & pDB262
RC   pAL19 from pBR322 & pDB262
RC   pEL11 from pAL7 & yeast 2 micron
RA   Okazaki K., Okazaki N., Kume K., Jinno S., Tanaka K., Okayama H.;
RT   "High-frequency transformation method and library transducing
RT   vectors for cloning mammalian cDNAs by trans-complementation of
RT   Schizosaccharomyces pombe";
RL   Nucleic Acids Res. 18:6485-6489(1990).
CC   Shares pBR322 sequences from the PvuII site to the EcoRI site with
CC   pcD.
CC   Plasmid linearized with PstI can be used to introduce cDNA libraries
CC   constructed in pcD or pcD2 vectors into Schizosaccharomyces pombe,
CC   resulting in the formation, by homologous recombination, of
CC   concatamers maintained as episomal DNA.
CC   Contains the ars sequence from Schizosaccharomyces pombe and the URA3
CC   gene from Saccharomyces cerevisiae.  Insertions at the PstI site
CC   inactivate ampicillin resistance.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--3.4, 1.1; HindIII--4.4; PstI--4.4. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAU9)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Schizosaccharomyces pombe)(E.coli)
CC   HO (Schizosaccharomyces pombe [ATCC 38436])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. yeast, ARS
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> pFL20 8000bp
FT                   1. pBR322 ScaI-PvuII 2581bp 3847..4361..2067, ori/tet
FT                   2. pFL20 PstI-PstI 1800bp, ARS/URA3 gene
FT                   -> pAU9 4400bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-ClaI-PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;