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ID   PAV2       preliminary; DNA; SYN; 8700 BP.
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AC   ATCC37216;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pAV2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAV1, pAV2 from Saccharomyces
RA   Wondisford F.E., Usala S.J., DeCherney G.S., Castren M., Radovick S.,
RA   Gyves P.W., Trempe J.P., Kerfoot B.P., Nikodem V.M., Carter B.J.,
RA   Laughlin C.A.;
RT   "Cloning of the human thyrotropin beta-subunit gene and transient
RT   expression of biologically active human thyrotropin after gene
RT   transfection";
RL   Mol. Endocrinol. 2:32-39(1988).
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RN   [2]
RC   pA11P.Xba from pA11P
RC   pAV1 from pA11P & AAV2
RC   pAV2 from pA11P.Xba & AAV2
RC   pCL93, pCL96 from pX1 & AAV2
RA   Laughlin C.A., Tratschin J.D., Coon H., Carter B.J.;
RT   "Cloning of infectious adeno-associated virus genomes in bacterial
RT   plasmids";
RL   Gene 23:65-73(1983).
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CC   Contains the complete viral genome for use in site-specific
CC   mutagenesis studies. Transfection into human 293 cells with an Ad
CC   helper virus results in infectious AAV virus particles.
CC   pAV1 (ATCC 37215) and pAV2 (ATCC 37216) both contain the complete
CC   AAV2 genome, but in opposite orientations.  The pAV2 vector has fewer
CC   polylinker sites than that of pAV1 (HindIII, ClaI, and one EcoRI site
CC   have been deleted). [2]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: C.A.Laughlin
CC   NM (pAV2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)(expression)(library)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pA11P remove small XbaI-XbaI, MCS
FT                   -> pA11P.Xba
FT                   1. pA11P.Xba BglII
FT                   2. AAV2 BglII 4700bp, complete
FT                   -> pAV2"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI virus AAV"
FT   promoter        0..0
FT                   /note="PRO virus AAV P5"
FT   promoter        0..0
FT                   /note="PRO virus AAV P19"
FT   promoter        0..0
FT                   /note="PRO virus AAV P40"
FT   terminator      0..0
FT                   /note="TER AAV polyadenylation"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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