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ID   PAVE1      preliminary; circular DNA; SYN; 2787 BP.
AC   ATCC37838;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pAVE1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pAVE1 from pUC19 & pCMVcat
RC   pAVE2 from pAVE1 & pMThGH111, hGH gene 3' end/polyA
RA   Ramirez-Solis R., Resendez-Perez D., Alvidrez-Quihui L.E.,
RA   Rincon-Limas D.E., Varela-Martinez R., Martinez-Rodriguez H.G.,
RA   Barrera-Saldana H.A.;
RT   "New vectors for the efficient expression of mammalian genes in
RT   cultured cells";
RL   Gene 87:291-294(1990).
RN   [2]
RC   plasmid from pCM5029 & pUC12
RC   pCMVcat from pBR322 & linker & plasmid & pRSVcat
RC   from pCMVcat
RC   from pRSVcat
RC   from pSV2cat
RA   Foecking M.K., Hofstetter H.;
RT   "Powerful and versatile enhancer-promoter unit for mammalian
RT   expression vectors";
RL   Gene 45:101-105(1986).
RN   [3]
RC   pUC12-hGHN from pUC12 & hGH gene
RC   pBR322::MT-1A from pBR322 & mouse MT-1 gene & linker
RC   pMThGH111 from pUC12-hGHN & pBR322::MT-1A
RC   pBX322 from pBR322 & lambda & linker
RC   pBR322::MT-1B from pBX322 & mouse MT-1 gene
RC   pMThGH112 from pUC12-hGHN & pBR322::MT-1B
RA   Palmiter R.D., Norstedt G., Gelinas R.E., Hammer R.E., Brinster R.L.;
RT   "Metallothionein-human GH fusion genes stimulate growth of mice";
RL   Science 222:809-814(1983).
CC   Shuttle expression vector.
CC   Constructed by ligating the larger HindIII/EcoRI fragment of pCMVcat
CC   (removing CAT and the SV40 polyadenylation signals) to the
CC   HindIII/EcoRI multiple cloning site of pUC19.
CC   Commercial use of the human cytomaglovirus promoter/enhancer has been
CC   patented on behalf of B. Fleckenstein and W. Schaffner by the
CC   Beringwerke.
CC   Interested parties should contact the patent holders before planning a
CC   commercial application.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--3.1; PstI--2.3, 0.8; XbaI--2.4, 0.7. (ATCC staff)
CC   The order of the major features in this plasmid is:
CC   hCMV enhancer/promoter - HindIII - MCS - EcoRI - bla - ori.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAVE1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)(monkey COS-7)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC8 PstI 2665bp 257..257
FT                   2. hCMV PstI-PstI, immediate early gene
FT                   -> pCM5029
FT                   1. pCM5029 ThaI-ThaI 619bp, CMV immediate-early prom
FT                   2. pUC12 HincII 2680bp 263..263
FT                   -> plasmid 3299bp
FT                   1. pBR322 AccI-AccI 2768bp 2246..4361..653
FT                   Klenow:Klenow
FT                   XbaI linker 6bp tctaga:XbaI linker 6bp tctaga
FT                   XbaI-EcoRI 2109bp 2251..4360, amp gene
FT                   2. plasmid XbaI-HindIII, CMV immediate-early promoter
FT                   3. pRSVcat HindIII-EcoRI 2300bp, cat gene
FT                   -> pCMVcat 5000bp
FT                   1. pUC19 EcoRI-HindIII 51bp 397..448, MCS
FT                   2. pCMVcat remove EcoRI-HindIII 2300bp,
FT                   \ pSV2cat EcoRI 4752.. to 3' cat
FT                   -> pAVE1 2787bp"
FT   misc_binding    0..0
FT                   /note="MCS BspEI-HindIII-SphI-SalI-BamHI-AvaI-SmaI-
FT                   KpnI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-SphI-SalI-BamHI-AvaI-SmaI-
FT                   KpnI-EcoRI-XmaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO hCMV"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;