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ID   PAVE2      preliminary; circular DNA; SYN; 3395 BP.
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AC   ATCC37839;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pAVE2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAVE1 from pUC19 & pCMVcat
RC   pAVE2 from pAVE1 & pMThGH111, hGH gene 3' end/polyA
RA   Ramirez-Solis R., Resendez-Perez D., Alvidrez-Quihui L.E.,
RA   Rincon-Limas D.E., Varela-Martinez R., Martinez-Rodriguez H.G.,
RA   Barrera-Saldana H.A.;
RT   "New vectors for the efficient expression of mammalian genes in
RT   cultured cells";
RL   Gene 87:291-294(1990).
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CC   Commercial use of the human cytomaglovirus promoter/enhancer has been
CC   patented on behalf of B. Fleckenstein and W. Schaffner by the
CC   Beringwerke.
CC   Interested parties should contact the patent holders before planning a
CC   commercial application.
CC   A shuttle vector for transient expression of cDNA inserts in mammalian
CC   cells.
CC   Constructed by inserting a 0.62 kb SmaI/EcoRI fragment from pMThGH
CC   containing part of exon 5 and 3' untranslated regions of the human
CC   growth hormone gene into the SmaI/EcoRI sites of pAVE1 (ATCC 37838).
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII-3.5; BamHI--3.5; EcoRI--3.5; SmaI--3.5. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pAVE2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)(monkey COS-7)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. hGH gene
FT                   3. MT-1 promoter
FT                   -> pMThGH111 6300bp
FT                   1. pAVE1 remove SmaI-EcoRI, MCS
FT                   2. pMThGH111 BamHI-EcoRI 2100bp, hGH gene
FT                   SmaI-EcoRI 620bp, hGH gene 3' end/polyA
FT                   -> pAVE2 3400bp"
FT   misc_binding    0..0
FT                   /note="MCS BspE1-HindIII-SphI-SalI-BamHI-AvaI-SmaI-
FT                   XmaI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-SphI-SalI-BamHI-AvaI-
FT                   SmaI-XmaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO hCMV"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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